PMID: 11328582May 1, 2001Paper

In vitro prevention of cell-mediated xeno-graft rejection via the Fas/FasL-pathway in CrmA-transducted porcine kidney cells

Xenotransplantation
M FujinoS Suzuki

Abstract

Cell-mediated cytotoxicity may be involved in delayed and/or chronic xenograft rejection in which apoptosis is induced in the grafted cells via the Fas/Fas-ligand (FasL) and perforin/granzyme pathways. One barrier to the potential use of xeonogenic grafts for humans may be Fas/FasL-mediated apoptosis, which would be blocked by the gene expression of cytokine response modifier A (CrmA), a cowpox virus gene product. The purpose of this study is to explore whether crmA is an effective candidate gene for inhibiting apoptosis in an in vitro model of xenograft rejection, using Fas-expressing non-primate cells cultured with a soluble recombinant human FasL (sFasL). A recombinant adenovirus vector expressing CrmA (AxCALNLCrmA) was successfully generated with a Cre-mediated switching system. PK15 cells, derived from a porcine kidney and infected with AxCALNLCrmA and/or AxCANCre at a multiplicity of infection (MOI) ranging from 0.1 to 100, were cultured with human sFasL derived from KFL74.18, a human FasL-overexpressed cell line. The gene-expression level of the PK15 cells was confirmed by CrmA-immune staining. Approximately 70% of the control PK15 cells showed induced apoptosis when cultured with sFasL. In contrast, the apoptosis was dr...Continue Reading

References

Jun 1, 1990·Human Immunology·D H Sachs, F H Bach
Sep 1, 1989·The Journal of Experimental Medicine·R N PiersonH Auchincloss
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D J PickupW K Joklik
Sep 29, 1995·The Journal of Biological Chemistry·M TewariV M Dixit
May 5, 1995·The Journal of Biological Chemistry·L T QuanG S Salvesen
Feb 11, 1994·Science·V GagliardiniJ Yuan
Mar 1, 1996·Nature Medicine·M TanakaS Nagata
Aug 1, 1996·Immunology Today·F H BachS C Robson
Jan 20, 1996·Human Gene Therapy·M HashimotoK Mikoshiba
Oct 18, 1996·Cell·E S AlnemriJ Yuan
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·S M SrinivasulaE S Alnemri
Mar 21, 1997·The Journal of Biological Chemistry·Q ZhouG S Salvesen
Apr 27, 1997·Transplantation·M J SmythJ A Trapani
May 2, 1998·The EMBO Journal·C ScaffidiM E Peter
Dec 16, 1998·Xenotransplantation·I FujiwaraT Oka
Apr 16, 1998·Trends in Biochemical Sciences·J WhisstockA M Lesk
Jun 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R A JonesG E Kass
Jan 5, 1999·The Journal of Experimental Medicine·G MacDonaldA H Greenberg
Feb 2, 1999·Experimental Cell Research·S C ChowG M Cohen
Feb 13, 1999·The Journal of Biological Chemistry·X M SunG M Cohen
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·D C HuangA Strasser

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Citations

Nov 19, 2003·Biochemical and Biophysical Research Communications·Mikiko KawasakiEiji Kobayashi
Apr 11, 2003·Molecular Immunology·Tadashi FurusawaTomoyuki Tokunaga
Aug 6, 2002·Current Opinion in Microbiology·Helen Everett, Grant McFadden
Apr 7, 2016·Medical Microbiology and Immunology·Mateusz KędziorJan Gutowicz
Mar 6, 2003·Plastic and Reconstructive Surgery·Brian R GastmanErnest K Manders

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