In vitro processing of transcripts containing novel tRNA-like sequences ('t-elements') encoded by wheat mitochondrial DNA

Plant Molecular Biology
P J Hanic-JoyceM W Gray

Abstract

We have recently described the properties of a wheat mitochondrial extract that is able to process, accurately and efficiently, artificial transcripts containing wheat mitochondrial tRNA sequences, with the production of mature tRNAs (P.J. Hanic-Joyce and M.W. Gray, J. Biol. Chem., in press). Such processing involves 5'-endonucleolytic, 3'-endonucleolytic, and tRNA nucleotidyltransferase activities. Here we show that this system also acts on transcripts containing sequences corresponding to an unusual class of short repeats ('t-elements') in wheat mtDNA. These repeats are theoretically capable of assuming a tRNA-like secondary structure, although stable transcripts corresponding to them are not detectable in vivo. We find that t-element sequences are processed with the same specificity and with comparable efficiency as are authentic tRNA sequences. Because known t-elements are located close to and in the same transcriptional orientation as active genes (18S-5S, 26S, tRNA(Pro)) in wheat mtDNA, our results raise the question of whether t-elements play a role in gene expression in wheat mitochondria.

References

Oct 23, 1987·Science·W H McClainS Altman
Jul 25, 1989·Nucleic Acids Research·E ZennaroM Bolotin-Fukuhara
Jul 14, 1989·Cell·G CarraraG P Tocchini-Valentini
Jul 25, 1989·Nucleic Acids Research·P B Joyce, M W Gray
Jan 1, 1980·Methods in Enzymology·A M Maxam, W Gilbert

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Citations

Oct 1, 1996·Plant Molecular Biology·S BinderA Brennicke
Jan 1, 1995·Molecular Biology Reports·A Marchfelder
Sep 4, 2008·Molecular Genetics and Genomics : MGG·Sophie Calixte, Linda Bonen
May 18, 2010·Nature Structural & Molecular Biology·Anthony GobertPhilippe Giegé
May 25, 1991·Nucleic Acids Research
Dec 30, 2015·The Plant Journal : for Cell and Molecular Biology·Birgit Stoll, Stefan Binder
Dec 20, 2011·Biochimie·Annette HölzleAnita Marchfelder
Feb 3, 2016·Physiologia Plantarum·Stefan BinderBirgit Stoll
Mar 6, 2021·The Plant Journal : for Cell and Molecular Biology·Thalia Salinas-GiegéLaurence Drouard

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