In vitro production of biotrophic-like cultures of Crinipellis perniciosa, the causal agent of witches' broom disease of Theobroma cacao

Current Microbiology
Lyndel W MeinhardtGonçalo Amarante Guimarães Pereira

Abstract

Witches' broom disease (WBD) of cacao, caused by the hemibiotrophic fungus, Crinipellis perniciosa, exhibits a succession of symptoms that are caused by the biotrophic phase of the fungus. However, the study of this biotrophic phase is limited by its exclusive growth inside the plant or in the presence of callus. Here we report for the first time a method for the growth and maintenance of the biotrophic-like phase of C. perniciosa on a defined medium with metabolites found in the diseased tissues. Our results suggest that glycerol is a key carbon source for this interaction. This is a crucial achievement toward understanding the biology of this fungus during the infectious phase of WBD.

References

Jul 27, 2001·BioTechniques·L W MeinhardtS M Tsai
May 9, 2003·Mycological Research·Maricília C C de ArrudaMaria Sueli S Felipe
Feb 1, 1962·Journal of Bacteriology·M MANDELSE T REESE
Jan 1, 1996·Annual Review of Phytopathology·L H Purdy, R A Schmidt
Apr 23, 2004·Molecular Microbiology·Jana KloseJames W Kronstad

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Citations

Apr 6, 2006·Current Microbiology·Dorival F FilhoMartin Brendel
Jun 7, 2008·Molecular Plant-microbe Interactions : MPMI·Johana RinconesGonçalo A G Pereira
Dec 9, 2008·Molecular Plant-microbe Interactions : MPMI·Cristina PungartnikAbelmon da Silva Gesteira
Oct 24, 2008·Phytopathology·Harry C Evans
Oct 15, 2010·Fungal Biology·Gildemberg A LealAntonio Figueira
Nov 21, 2008·Molecular Plant Pathology·Lyndel W MeinhardtGonçalo A G Pereira
Oct 2, 2012·Fungal Genetics and Biology : FG & B·Bruno V de OliveiraGonçalo A G Pereira
Oct 22, 2013·Fungal Genetics and Biology : FG & B·Heliana Argôlo Santos CarvalhoFabienne Micheli
Dec 30, 2014·Journal of Experimental Botany·Joan BarauGonçalo Amarante Guimarães Pereira
Aug 6, 2009·BMC Microbiology·Acássia B L PiresGonçalo A G Pereira
Dec 2, 2017·Molecular Plant Pathology·Bryan A BaileyLyndel W Meinhardt
Jun 15, 2007·Annals of Botany·Abelmon S GesteiraJúlio C M Cascardo
May 23, 2019·Phytopathology·Jean-Philippe MarelliAlina S Puig
Mar 13, 2014·Molecular Plant Pathology·Bryan A BaileyLyndel Meinhardt
Mar 21, 2020·International Journal of Biological Macromolecules·Edson Mario de Andrade SilvaFabienne Micheli

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