In vitro Production of IL-6 and IFN-γ is Influenced by Dietary Variables and Predicts Upper Respiratory Tract Infection Incidence and Severity Respectively in Young Adults

Frontiers in Immunology
Huicui MengConnie J Rogers

Abstract

Assessment of immune responses in healthy adults following dietary or lifestyle interventions is challenging due to significant inter-individual variability. Thus, gaining a better understanding of host factors that contribute to the heterogeneity in immunity is necessary. To address this question, healthy adults [n = 36, 18-40 years old, body mass index (BMI) 20-35 kg/m(2)] were recruited. Dietary intake was obtained via 3-day dietary recall records, physical activity level was evaluated using the International Physical Activity Questionnaire, and peripheral blood mononuclear cells were isolated from peripheral blood. Expression of activation markers on unstimulated immune subsets was assessed by flow cytometry. T-cell proliferation and cytokine secretion was assessed following in vitro stimulation with anti-CD3 or lipopolysaccharide. Furthermore, the incidence and severity of cold or flu symptoms were obtained from self-reported upper respiratory tract infection (URTI) questionnaires. The relationship between activation marker expression on T cells and T-cell effector functions; and in vitro cytokine secretion and URTI was determined by linear or logistic regression. CD69 and CD25 expression on unstimulated T cells was signif...Continue Reading

References

Jun 1, 1990·The Journal of Investigative Dermatology·W A Kuziel, W C Greene
Dec 1, 1990·International Journal of Sports Medicine·D C NiemanK Arabatzis
Feb 1, 1990·Journal of Steroid Biochemistry·A H Schuurs, H A Verheul
Feb 1, 1989·European Journal of Immunology·A RissoA Bargellesi
Mar 1, 1983·The American Journal of Clinical Nutrition·C BouchardG Thériault
Feb 1, 1994·Medicine and Science in Sports and Exercise·D C Nieman
Jun 1, 1997·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·R B Turner
Aug 1, 1997·The American Journal of Clinical Nutrition·N S Scrimshaw, J P SanGiovanni
Jan 7, 1998·Immunological Reviews·I A RamshawJ C Ruby
Jul 14, 2000·Physiological Reviews·B K Pedersen, L Hoffman-Goetz
Mar 12, 2002·European Journal of Clinical Investigation·Günter Weiss
Jun 19, 2002·European Journal of Applied Physiology·Panagiota KlentrouMichael Plyley
Dec 24, 2002·The British Journal of Nutrition·Philip C Calder, Samantha Kew
May 16, 2003·JAMA : the Journal of the American Medical Association·Aram V ChobanianUNKNOWN National High Blood Pressure Education Program Coordinating Committee
Jul 26, 2003·Folia Microbiologica·M Ferencík, L Ebringer
Aug 12, 2003·Journal of Applied Physiology·Thomas J CieslakPanagiota Klentrou
Oct 4, 2003·Journal of Leukocyte Biology·Kate SchroderDavid A Hume
Dec 31, 2003·Trends in Immunology·Paresh DandonaArindam Bandyopadhyay
Jan 30, 2004·Nature Immunology·Phyllis Jean Linton, Kenneth Dorshkind
Sep 24, 2005·The British Journal of Nutrition·Ruud AlbersBernhard Watzl
Oct 31, 2006·The American Journal of Medicine·Jessica ChubakCornelia M Ulrich
Feb 3, 2007·Expert Reviews in Molecular Medicine·Rania D KovaiouBeatrix Grubeck-Loebenstein
Jun 16, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Kenneth W HanceJohn W Greiner
Dec 25, 2007·The Journal of Nutrition·Connie J RogersStephen D Hursting
Sep 23, 2008·Clinical Journal of Sport Medicine : Official Journal of the Canadian Academy of Sport Medicine·Amanda J CoxPeter A Fricker
Jun 1, 2000·Nutrition Research Reviews·P C Calder, A A Jackson
Apr 23, 2010·International Immunology·Tadamitsu Kishimoto
Apr 27, 2010·The Lancet Infectious Diseases·Tracy Hussell, John Goulding
Feb 8, 2011·Biochimica Et Biophysica Acta·Jürgen SchellerStefan Rose-John
Mar 10, 2011·Scandinavian Journal of Medicine & Science in Sports·M GleesonA S Muhamad
Jun 12, 2012·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A C de AlbuquerqueN A P Nogueira
Nov 3, 2012·PloS One·Elisenda Alari-PahissaPilar Lauzurica
May 15, 2013·The Proceedings of the Nutrition Society·Philip C Calder
Mar 29, 2014·Annals of the New York Academy of Sciences·Margherita T Cantorna, Amanda Waddell

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Methods Mentioned

BETA
flow cytometry
blood draw
biosensor
ELISA

Software Mentioned

FlowJo
Statistical Analysis System ( SAS
Food Processor SQL
GraphPad Prism

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