In vitro production of panton-valentine leukocidin among strains of methicillin-resistant Staphylococcus aureus causing diverse infections

Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America
Stephanie M HamiltonDennis L Stevens

Abstract

Community-acquired methicillin-resistant Staphylococcus aureus strains have recently been associated with severe necrotizing infections. Greater than 75% of these strains carry the genes for Panton-Valentine leukocidin (PVL), suggesting that this toxin may mediate these severe infections. However, to date, studies have not provided evidence of toxin production. Twenty-nine community-acquired methicillin-resistant Staphylococcus aureus and 2 community-acquired methicillin-susceptible S. aureus strains were collected from patients with infections of varying severity. Strains were analyzed for the presence of lukF-PV and SCCmecA type. PVL production in lukF-PV gene-positive strains was measured by ELISA, and the amount produced was analyzed relative to severity of infection. Only 2 of the 31 strains tested, 1 methicillin-resistant Staphylococcus aureus abscess isolate and 1 nasal carriage methicillin-susceptible S. aureus isolate, were lukF-PV negative. All methicillin-resistant Staphylococcus aureus strains were SCCmec type IV. PVL was produced by all strains harboring lukF-PV, although a marked strain-to-strain variation was observed. Twenty-six (90%) of 29 strains produced 50-350 ng/mL of PVL; the remaining strains produced PVL...Continue Reading

References

Jan 1, 1989·Reviews of Infectious Diseases·G J Dammin
Jan 1, 1995·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·M C LaytonJ E Patterson
May 14, 1998·Microbiology·Lihong ZhangLars Frykberg
Aug 26, 1998·The New England Journal of Medicine·F D Lowy
Oct 19, 1999·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·G LinaJ Etienne
Aug 31, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·T S NaimiK E Smith
Sep 18, 2001·JAMA : the Journal of the American Medical Association·A V GroomJ E Cheek
Jun 1, 2002·The Journal of Antimicrobial Chemotherapy·Eileen M Graffunder, Richard A Venezia
Sep 14, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Philippe DufourHervé Richet
Oct 24, 2002·The Pediatric Infectious Disease Journal·Kevin Purcell, Jaime E Fergie
Jun 28, 2003·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Andrew L CampbellGary S Marshall
Dec 11, 2003·JAMA : the Journal of the American Medical Association·Timothy S NaimiRuth Lynfield
Jan 15, 2004·The Journal of Infectious Diseases·Valérie GauduchonGerard Lina
Dec 23, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·John S FrancisJohn G Bartlett
Apr 9, 2005·The New England Journal of Medicine·Scott K FridkinUNKNOWN Active Bacterial Core Surveillance Program of the Emerging Infections Program Network
Apr 9, 2005·The New England Journal of Medicine·Loren G MillerBrad Spellberg
May 19, 2006·Emerging Infectious Diseases·Jeffrey C HagemanL Clifford McDonald
Aug 18, 2006·The New England Journal of Medicine·Gregory J MoranUNKNOWN EMERGEncy ID Net Study Group
Sep 20, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Pia S PannarajSheldon L Kaplan
Jan 20, 2007·Science·Maria Labandeira-ReyM Gabriela Bowden

❮ Previous
Next ❯

Citations

Jan 24, 2014·International Journal of Inflammation·Shashi Prakash MishraSanjeev Kumar Gupta
May 10, 2008·Critical Care : the Official Journal of the Critical Care Forum·Hongmei GaoSimon J Finney
Sep 15, 2009·Applied and Environmental Microbiology·Avanish K VarshneyBettina C Fries
Aug 30, 2008·The Journal of Infectious Diseases·William M Nauseef
May 24, 2013·The Journal of Infectious Diseases·Tanyalak ParimonAmy E Bryant
Jan 26, 2016·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·John H HwangLaura E Crotty Alexander
Mar 8, 2013·Current Opinion in Microbiology·Pauline Yoong, Victor J Torres
Oct 30, 2012·The Lancet Infectious Diseases·Laura J ShallcrossAndrew C Hayward
Apr 17, 2009·Medicina clínica·Mónica Ibáñez BarcelóSantos Castañeda
May 5, 2009·International Journal of Antimicrobial Agents·Fadi BittarJean-Marc Rolain
Oct 20, 2010·Pediatric Annals·David R Berk, Susan J Bayliss
Aug 23, 2008·Journal of Microbiological Methods·Kanenari OishiKeiichi Hiramatsu
Jan 27, 2009·The Mount Sinai Journal of Medicine, New York·Rupali K DoshiFrances Wallach
Jun 4, 2008·Expert Opinion on Pharmacotherapy·Edina Avdic, Sara E Cosgrove
May 17, 2014·Scandinavian Journal of Infectious Diseases·Eunice Mireya Borbón-EsquerMariana Del Rocío Ruiz-Briseño
Jun 2, 2015·The Journal of Infection·Claire E Turner, Shiranee Sriskandan
Dec 3, 2008·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C BadiouG Lina
Sep 18, 2016·The Journal of Infectious Diseases·David M Bamberger
May 23, 2014·Microbiology and Molecular Biology Reviews : MMBR·Francis Alonzo, Victor J Torres
May 3, 2011·Critical Care Medicine·Jeffrey S Ustin, Mark A Malangoni
Nov 6, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Natalia MalachowaFrank R DeLeo
Dec 14, 2016·Open Forum Infectious Diseases·Trine A KnudsenUNKNOWN Danish Staphylococcal Bacteremia Study Group
Jun 23, 2020·The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien Des Maladies Infectieuses Et De La Microbiologie Médicale·Ahmed AlbarragAmani Al-Amodi
Dec 12, 2020·Infectious Disease Clinics of North America·Timothy J Hatlen, Loren G Miller
Mar 16, 2019·Microbiology Spectrum·Kayan Tam, Victor J Torres
May 29, 2015·Clinical Microbiology Reviews·Steven Y C TongVance G Fowler

❮ Previous
Next ❯

Related Concepts

Related Feeds

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.