In vitro production of Sudanese camel (Camelus dromedarius) embryos from epididymal spermatozoa and follicular oocytes of slaughtered animals

Polish Journal of Veterinary Sciences
A E AbdelkhalekM H Farouk

Abstract

Application of assisted reproductive technology in camelidea, such as artificial insemination (AI) and embryo transfer, has been slow in comparison to that for other livestock species. In Egypt, there are few attempts to establish in vitro maturation (IVM) and fertilization (IVF) techniques in dromedary camel. The present study was carried out to produce Sudanese camel embryos using in vitro matured oocytes and epididymal spermatozoa. Dromedary camel ovaries were collected from abattoirs and then, the oocytes were aspirated from all the visible follicles on the ovarian surface (~2-8 mm in a diameter). Meanwhile, Fetal Dromedary Camel Serum (FDCS) was obtained from camel fetuses after slaughtering. Thereafter, only Cumulus Oocyte Complexes (COCs) were matured in vitro in the Tissue Culture Medium (TCM-199) complemented with 10% FDCS. Spermatozoa required for in vitro fertilization were collected from testes (epididymal cauda) of the slaughtered camel bulls. The results clearly showed that the maturation rate of oocytes at metaphase II was about 59.5% while the fertilization rate was around 70.4%. Intriguingly, the embryo rates determined were 13.1%, in 2-cell; 0.0%, in 4-cell; 34.7%, in 8-16% cell; 39.1%, in morula and 13.1% in ...Continue Reading

References

Apr 16, 2003·Animal Reproduction Science·Aminu DeenM S Sahani
Nov 1, 2003·Cloning and Stem Cells·Marina J SansinenaRobert A Godke
Jun 9, 2004·Journal of Ethnopharmacology·Joseph TaiDavid Hasman
Sep 1, 2005·Zygote : the Biology of Gametes and Early Embryos·Esther VelillaMaria-Teresa Paramio
Apr 1, 1986·Theriogenology·J J ParrishN L First

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Citations

Apr 30, 2020·Animals : an Open Access Journal From MDPI·Hammed A TukurIslam M Saadeldin
Feb 15, 2019·The Journal of Reproduction and Development·Islam M SaadeldinAbdullah N Alowaimer
Jan 8, 2021·Tropical Animal Health and Production·Mohamed A ShahinMostafa A El-Harairy

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