Abstract
Human natural killer (NK) cells expressing the HNK-1 differentiation antigen were established in long-term tissue culture for over 3 months. The fluorescence-activated cell sorter-purified HNK-1+ cells required both phytohemagglutinin and exogenous interleukin 2 to propagate in long-term culture. After 2 weeks of culture, virtually all of the growing cells exhibited the surface membrane phenotype associated with immature HNK-1+ cells, since they simultaneously expressed the HNK-1, Leu-4 and Leu-2a but lacked the M1. Leu-3a and T6 antigens, and Fc gamma receptors. They exhibited a lymphoblastoid appearance, contained cytoplasmic granules, and exhibited spontaneous cytotoxic function against a broader spectrum of target cells than did fresh HNK-1+ cells from the same donor. Cultured HNK-1+ cells lacked antibody-dependent cell-mediated cytotoxic (ADCC) function, while fresh HNK-1+ were fully capable of ADCC function. On the other hand, cultured HNK-1- cells were lymphoblasts without cytoplasmic granules or NK cytotoxic function. The cultured HNK-1+ cells gradually lost their HNK-1 antigen expression over time, although the expression of other surface antigens (e.g., Leu-4 and Leu-2a) was unchanged. With prolonged culture (greater ...Continue Reading
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