In vitro QuantiFERON-TB gold antigen specific interleukin-1beta to diagnose TB among HIV-positive subjects

Tuberculosis
Maddineni PrabhavathiAlamelu Raja

Abstract

The recently introduced IFN-γ release assay (IGRA) has been reported to improve the diagnosis of TB. However, IGRA has suboptimal sensitivity to diagnose TB among HIV co-infected subjects. Apart from IFN-γ, the pro inflammatory cytokines such as Interleukin-1beta (IL-1β), Tumor necrosis factor-alpha (TNF-α), IL-2, IL-6, IL-8 and IL-12 are also play a major role in mycobacterial infections. This study aimed to analyze these cytokines for detecting active TB among HIV sero positive subjects. We had prospectively enrolled 53 HIV positive subjects and 55 HIV-TB co-infected patients from India. IGRA was performed by using QuantiFERON TB-Gold In tube (QFT-GIT) method. TB antigen specific IL-1β, TNF-α, IL-2, IL-6, IL-8 and IL-12 levels were evaluated by ELISA in plasma harvested from QFT-GIT tubes. The TB antigen specific IL-1β levels were significantly elevated in HIV-TB co-infected patients compared to HIV positive subjects (p = 0.0004). The specificity of both IL-1β (50.94%) and QFT-GIT (52.83%) remained similar in HIV positive subjects (p = 0.24). However, IL-1β had shown higher sensitivity (72.73%) than QFT-GIT (54.55%) to diagnose TB among HIV co-infected patients. Moreover, in culture test positive HIV-TB patients, antigen spec...Continue Reading

References

Apr 1, 1996·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·R W Shafer, B R Edlin
May 30, 2009·PloS One·Basirudeen Syed Ahamed KabeerAlamelu Raja
Mar 23, 2011·Lancet·Stephen D Lawn, Alimuddin I Zumla

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Citations

Jul 3, 2016·Clinica Chimica Acta; International Journal of Clinical Chemistry·Balaji PathakumariAlamelu Raja
Dec 2, 2019·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Guangming ChenYanhong Wang

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