PMID: 9537859Apr 16, 1998Paper

In vitro reactivity of cryoglobulin IgM and IgG in hepatitis C virus-associated mixed cryoglobulinemia

Journal of Hepatology
P SchottH Hartmann

Abstract

Mixed cryoglobulinemia is frequently associated with chronic hepatitis C virus infection. We aimed to clarify the mechanism, kinetics and participating proteins in cryoprecipitate formation, which are still being debated. Eighteen patients with cryoglobulinemia were studied. Isolated serum cryoprecipitates and purified cryoglobulin IgM and IgG fractions were analyzed in vitro by turbidimetry for temperature-dependent complex formation. Immunoglobulin reactivity, i.e. in cryoprecipitates and in cryoglobulin-free sera, was studied using immunoblot and enzyme immunoassays. HCV RNA was detected by reverse transcriptase/polymerase chain reaction. By turbidimetry, purified cryo-IgM precipitated (in the absence of HCV RNA) with cryo-IgG as well as with non-cryoglobulin IgG and with IgG Fc or F(ab')2 fragments. In contrast, purified cryo-IgG did not precipitate with non-cryoglobulin IgM. Anti-HCV IgG reactivity was found in cryoglobulin-free sera, in cryoprecipitates and in purified cryoglobulin IgG fractions. The respective titers were similar. Purified cryo-IgM did not react to HCV-encoded proteins. Binding of cryo-IgM to heterologous IgG was inhibited by intact IgG (up to a mean of about 52%) as well as by IgG Fc (33%) and F(ab')2 f...Continue Reading

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Citations

Nov 1, 2001·Clinics in Liver Disease·S MehtaH L Bonkovsky
Sep 8, 2012·Clinical & Developmental Immunology·Peter D Gorevic
Oct 16, 2004·Liver International : Official Journal of the International Association for the Study of the Liver·José A Girón-GonzálezLeopoldo Martín-Herrera
Apr 23, 2013·International Journal of Hepatology·Jun Murakami, Yukihiro Shimizu
May 3, 2016·World Journal of Hepatology·Shilu MathewIshtiaq Qadri
Jan 16, 2003·Seminars in Arthritis and Rheumatism·Leslie E WilsonPeter D Gorevic
Mar 2, 1999·Archives of Pathology & Laboratory Medicine·U Kallemuchikkal, P D Gorevic
Jan 8, 2000·Hematology/oncology Clinics of North America·A Dispenzieri, P D Gorevic
Aug 28, 2021·World Journal of Gastroenterology : WJG·Jessica FaccioliLorenzo Ridola

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