In vitro receptor binding properties of a "painless" NGF mutein, linked to hereditary sensory autonomic neuropathy type V.

Biochemical and Biophysical Research Communications
Sonia CovaceuszachAntonino Cattaneo

Abstract

Nerve Growth Factor (NGF) signalling is mediated by the TrkA and p75NTR receptors. Besides its neurotrophic and survival activities, NGF displays a potent pro-nociceptive activity. Recently, a missense point mutation was found in the NGFB gene (C661T, leading to the aminoacid substitution R100W) of individuals affected by a form of hereditary loss of pain perception (hereditary sensory and autonomic neuropathy type V, HSAN V). In order to gain insights into the functional consequences of the HSAN V NGF mutation, two sets of hNGFR100 mutants were expressed in Escherichia coli and purified, as mature NGF or proNGF, for in vitro receptor binding studies. Here, we show by Surface Plasmon Resonance analysis that the R100 mutation selectively disrupts binding of hNGF to p75NTR receptor, to an extent which depends on the substituting residue at position 100, while the affinity of hNGFR100 mutants for TrkA receptor is not affected. As for unprocessed hproNGF, the binding of the R100 variants to p75NTR receptor shows only a limited impairment, showing that the impact of the R100 mutation on p75NTR receptor binding is greater in the context of mature, processed hNGF. These results provide a basis for elucidating the mechanisms underlying...Continue Reading

References

Nov 21, 1986·Cell·D JohnsonM Chao
Jun 1, 1983·Brain : a Journal of Neurology·P J DyckE H Lambert
Dec 1, 2001·Science·R LeeB L Hempstead
Aug 8, 1952·Annals of the New York Academy of Sciences·R LEVI-MONTALCINI
Feb 21, 2004·Human Molecular Genetics·Elisabet EinarsdottirMonica Holmberg
May 19, 2004·FEBS Letters·Marco KliemannelElisabeth Schwarz
Sep 27, 2005·Neurobiology of Disease·Nathalie VerpoortenVincent Timmerman
Jun 17, 2006·Annual Review of Neuroscience·Sophie Pezet, Stephen B McMahon
Aug 31, 2006·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Louis F Reichardt
Nov 17, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Koichi ObataKoichi Noguchi
Mar 7, 2007·Molecular Interventions·Grant D Nicol, Michael R Vasko
Aug 8, 2007·Journal of Molecular Biology·Evgeny Krissinel, Kim Henrick
Oct 16, 2007·Nature Neuroscience·Pernille JansenAnders Nykjaer
May 24, 2008·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·D C de AndradeM J Teixeira
Aug 19, 2008·Journal of Neuroscience Research·Tomoko WatanabeToshihide Yamashita
Oct 28, 2008·Journal of Alzheimer's Disease : JAD·Antonino CattaneoFrancesca Paoletti
Dec 18, 2008·Proteins·Francesca PaolettiDoriano Lamba
Apr 10, 2009·Current Alzheimer Research·Sonia CovaceuszachAntonio Cattaneo

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Citations

Sep 4, 2012·Molecular Neurobiology·Antonino Cattaneo, Pietro Calissano
Apr 17, 2010·Cell Death and Differentiation·P CalissanoN Canu
Nov 30, 2012·Journal of Translational Medicine·Luigi AloeLuigi Manni
Oct 3, 2012·Journal of Pain Research·Vinayak Kumar, Brandon A Mahal
Jan 21, 2015·International Journal of Molecular Sciences·Laura MarchettiAntonino Cattaneo
May 31, 2011·Expert Opinion on Drug Delivery·Francesca MalerbaAntonino Cattaneo
Feb 6, 2014·The European Journal of Neuroscience·Simona Capsoni
Feb 6, 2014·The European Journal of Neuroscience·Gary R Lewin, Anders Nykjaer
Mar 19, 2013·European Journal of Pharmacology·Shafaq SikandarAnthony H Dickenson
Dec 30, 2016·Brain : a Journal of Neurology·Simona CapsoniAntonino Cattaneo
Jan 25, 2012·Nature Reviews. Neurology·Annelies RotthierKatrien Janssens
Apr 21, 2021·SLAS Discovery·Francesca MalerbaAntonino Cattaneo
Jul 28, 2013·Journal of Cell Science·Laura MarchettiAntonino Cattaneo
May 18, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Giovanna TestaSimona Capsoni

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