In vitro reconstitution and analysis of mononucleosomes containing defined DNAs and proteins

Methods : a Companion to Methods in Enzymology
J J Hayes, K M Lee

Abstract

Increasingly, biochemical analyses of processes that occur within eukaryotic nuclei such as transcription and replication require the construction of specific chromatin substrates. Nucleosome complexes reconstituted in vitro have been key elements in a variety of recent studies of polymerase progression and trans-acting factor binding activities. Reconstituted complexes can be easily constructed from purified components in quantities suitable for biochemical and biophysical studies. In addition, reconstituted mononucleosome complexes exhibit native biochemical and biophysical properties but necessarily contain much less heterogeneity with regard to both protein and DNA components than bulk complexes isolated from natural sources. This review details the protocols for reconstitution of model mononucleosome complexes that contain unique DNA sequences and specifically tailored core histone proteins and describes common pitfalls associated with these procedures.

References

Jun 26, 1979·Biochemistry·K Tatchell, K E Van Holde
Oct 1, 1992·Scientific American·M Grunstein
Aug 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J J HayesA P Wolffe
Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J J Hayes, A P Wolffe
Jan 1, 1991·Progress in Nucleic Acid Research and Molecular Biology·R T Simpson
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J J HayesA P Wolffe
Nov 5, 1990·Journal of Molecular Biology·T E Shrader, D M Crothers
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A P Wolffe, H R Drew
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T E Shrader, D M Crothers
May 1, 1987·Molecular and Cellular Biology·J M Gottesfeld
Feb 20, 1986·Journal of Molecular Biology·D J Clark, J O Thomas
Dec 20, 1985·Journal of Molecular Biology·H R Drew, A A Travers
Sep 11, 1981·Nucleic Acids Research·A Klug, L C Lutter
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R T Simpson, D W Stafford
Mar 29, 1984·Biochimica Et Biophysica Acta·I O Walker, A P Wolffe
Nov 25, 1981·Nucleic Acids Research·D L Bates, J O Thomas
Dec 25, 1980·Nature·J AllanF X Aviles
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·J J HayesA P Wolffe
Mar 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·L L WallrathS C Elgin
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J J Hayes, A P Wolffe
Mar 22, 1996·Journal of Molecular Biology·A HamicheA Prunell
Jan 1, 1996·Critical Reviews in Eukaryotic Gene Expression·T M Fletcher, J C Hansen
Nov 1, 1974·Nucleic Acids Research·M Noll

❮ Previous
Next ❯

Citations

Aug 19, 1997·Proceedings of the National Academy of Sciences of the United States of America·K M Lee, J J Hayes
Nov 26, 2009·The Journal of Biological Chemistry·Hope A ColeJeffrey J Hayes
Nov 6, 2009·The Journal of Biological Chemistry·Geetaram SahuAkhilesh K Nagaich
Oct 18, 2000·The EMBO Journal·D R ChafinJ J Hayes
Feb 11, 2005·Nucleic Acids Research·Prasad N KuduvalliNancy L Craig
Dec 7, 2010·Nucleic Acids Research·Manu Shubhdarshan ShuklaStefan Dimitrov
Jan 1, 2004·Molecular and Cellular Biology·Joseph M VitoloJeffrey J Hayes
Jan 24, 2006·Molecular and Cellular Biology·Cécile-Marie DoyenStefan Dimitrov
Aug 12, 2005·Cytogenetic and Genome Research·J YeT H Eickbush
Nov 5, 2008·PloS One·Pauline DupaigneEric Le Cam
Nov 15, 2003·Current Biology : CB·Zhenbo HanJill M Schumacher
May 2, 2002·Chemistry & Biology·Megan E NúñezJacqueline K Barton
Jul 14, 2012·PloS One·Daria GaykalovaJoseph A Califano
Nov 2, 2016·Nucleic Acids Research·He FangJeffrey J Hayes
Nov 10, 2018·Genome Research·Xinyang Yu, Michael J Buck
Jan 18, 2006·Nature Chemical Biology·John D TrzupekDale L Boger
Jan 20, 2007·The Journal of Biological Chemistry·Zungyoon YangJeffrey J Hayes
Aug 23, 2007·The Journal of Biological Chemistry·Xiaodong Wang, Jeffrey J Hayes
Apr 17, 2003·The Journal of Biological Chemistry·Chunyang Zheng, Jeffrey J Hayes
Sep 27, 2003·The Journal of Biological Chemistry·Aruna RamachandranGavin R Schnitzler
Mar 30, 2011·Journal of the Science of Food and Agriculture·Santiago Gallegos-TintoréJavier Vioque
Feb 10, 2007·The Journal of Biological Chemistry·Steven A Roberts, Dale A Ramsden
Sep 7, 2000·The Journal of Biological Chemistry·H ShirakawaY Postnikov
Jul 23, 2004·The Journal of Biological Chemistry·Dimitar AngelovStefan Dimitrov
Feb 12, 2008·Current Protocols in Molecular Biology·G R Schnitzler

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.