In vitro reconstitution of fibrillar collagen type I assemblies at reactive polymer surfaces

Biomacromolecules
Katrin SalchertCarsten Werner

Abstract

The reconstitution of fibrillar collagen and its assemblies with heparin and hyaluronic acid was studied in vitro. Fibril formation kinetics were analyzed by turbidity and depletion measurements in solutions containing varied concentrations of collagen and glycosaminoglycans. Fibril-forming collagen solutions were further applied for the coating of planar substrates which had been modified with alternating maleic anhydride copolymer films before. The immobilized collagen assemblies were characterized with respect to the deposited amount of protein using ellipsometry and acidic hydrolysis/HPLC-based amino acid analysis, respectively. AFM, SEM, and cLSM were utilized to gain information on structural features and patterns formed by surface-attached fibrils depending on the initial solution concentrations of collagen. The results revealed that the addition of heparin and hyaluronic acid affected both the fibril dimensions and the meshwork characteristics of the surface-bound fibrils.

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Citations

Jun 2, 2005·Journal of Materials Science. Materials in Medicine·K SalchertC Werner
Aug 27, 2011·Biomacromolecules·Sylvie LéluCatharina de Lange Davies
Jun 17, 2008·Nature Methods·Kristin AlbertiPeter W Zandstra
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Jan 10, 2016·Breast Cancer Research : BCR·James C McConnellCharles H Streuli
Jul 9, 2008·Biomaterials·Babette LanferCarsten Werner
Oct 31, 2009·Electrophoresis·Lucia D'UlivoMarja-Liisa Riekkola
Aug 18, 2005·Journal of Biomedical Materials Research. Part a·Hae-Won KimHyoun-Ee Kim
Jan 20, 2007·Journal of Biomedical Materials Research. Part a·Martin T MoranYuri Rochev
Sep 17, 2005·Stem Cells·Joachim OswaldMartin Bornhäuser
Jul 20, 2011·Biotechnology and Bioengineering·Nuno Miranda CoelhoGeorge Altankov
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Jul 30, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Tighe A SpurlinAnne L Plant

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