PMID: 7334371Dec 1, 1981Paper

In vitro release of endogenous amino acids from granule cell-, stellate cell-, and climbing fiber-deficient cerebella

Journal of Neurochemistry
R S FlintW J McBride

Abstract

The K+-stimulated, Ca2+-dependent release of glutamate, aspartate, gamma-aminobutyric acid (GABA), alanine, taurine, and glycine was measured in slices of cerebella obtained from control, and granule cell-, granule cell plus stellate cell-, or climbing fiber-deficient cerebella of the rat. The 55 mM-K+-stimulated release of glutamate and GABA was 10-fold greater in the presence of Ca2+ than in its absence. The stimulated release of aspartate was 4-fold higher when Ca2+ was present in the bathing media, while the value for alanine was twice as high as the amount obtained in the absence of Ca2+. There was no stimulated release of either taurine or glycine from the cerebellar slices. Increasing the Mg2+ concentration to 16 mM inhibited the K+-stimulated, Ca2+-dependent release of glutamate, GABA, aspartate, and alanine 85% or more. The K+-stimulated, Ca2+ dependent release of glutamate, aspartate, and alanine from x-irradiated cerebella deficient in granule cells was reduced to 50-57% of control value. Additional x-irradiation treatment, which further reduced the cerebellar granule cell population and also prevented the acquisition of stellate cells, decreased the release of glutamate by 77%, aspartate by 66%, alanine by 91%, and,...Continue Reading

References

Jan 1, 1978·Neuroscience·M E Sandoval, C W Cotman
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Citations

Dec 8, 1992·European Journal of Pharmacology·B G Dickie, J A Davies
Jan 1, 1989·Progress in Neurobiology·R J Huxtable
Jan 1, 1992·Progress in Neurobiology·P Saransaari, S S Oja
May 1, 1985·Brain Research Bulletin·J M MurphyW J McBride
Aug 1, 1985·Brain Research Bulletin·R S FlintW J McBride
Jan 1, 1987·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·F AngelatouE D Kouvelas
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·V GalloG Levi
Jan 1, 1990·Annals of the New York Academy of Sciences·M RaiteriP Paudice
Sep 1, 1984·Journal of Neurochemistry·M G Larrabee
Oct 1, 1988·Journal of Neurochemistry·C Fischer-BovenkerkT Ueda
May 1, 1983·Journal of Neurochemistry·M E FreemanJ E Smith
Jan 20, 1986·Life Sciences·R S Flint, W J McBride
Jan 1, 1987·Journal of Neuroscience Research·S S Oja, P Kontro
Jan 1, 1984·Journal of Neuroscience Research·C Beas-ZarateA Feria-Velasco

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