In vitro release of hypothalamic beta-endorphin (beta E) by arginine vasopressin, corticotropin-releasing hormone and 5-hydroxytryptamine: evidence for release of opioid active and inactive beta E forms

Neuropeptides
D M Bronstein, H Akil

Abstract

The aims of the present experiments were: 1) to test whether substances which modulate beta-endorphin-immunoreactive (beta E-ir) release from the pituitary gland might act similarly in hypothalamic tissue; and 2) to further characterize the beta E-ir forms which are released from hypothalamus. To address these questions, hypothalamic tissue was incubated in vitro for 10 min periods in either normal media (basal conditions) or in media containing 55 mM KCl or one of several other test substances (stimulation conditions) and release was estimated by measuring the beta E-ir concentrations in the media. Depolarizing concentrations of K+ increased beta E-ir release 2-3 fold over basal levels and this effect appeared to be Ca2(+)-dependent. Dose-dependent increases in beta E-ir release were elicited by nanonolar to micromolar concentrations of either corticotropin-releasing hormone (CRH), arginine vasopressin (AVP), or 5-hydroxytryptamine (5-HT). Conversely, dopamine (1 microM) inhibited both the basal and K(+)-stimulated release of beta E-ir from hypothalamus. Gel filtration chromatography revealed that beta E1-31 and beta E1-27/beta E1-26 were the primary beta E-ir peptides released under either basal or CRH-stimulated conditions; ...Continue Reading

References

May 15, 1979·European Journal of Pharmacology·H OsborneA Herz
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·P NicolasC H Li
Jun 24, 1982·Brain Research·A BiegonB S McEwen

Citations

Jul 8, 2008·Psychopharmacology·Judith E GriselVictoria L Turgeon
Apr 24, 1992·Brain Research. Developmental Brain Research·L P Kapcala, C F Weng
Nov 1, 1992·Brain Research Bulletin·L P KapcalaH H Juang
Aug 22, 2002·Progress in Neuro-psychopharmacology & Biological Psychiatry·Chul Na, Young-Sik Lee
Aug 1, 1991·Alcoholism, Clinical and Experimental Research·M L Adams, T J Cicero

Related Concepts

Beta-endorphin (1-27)
Metazoa
Argipressin Tannate
Beta-Endorphin
Ampholytes
Corticotropin-Releasing Hormone
Dose-Response Relationship, Drug
Organum Vasculosum Laminae Terminalis
Peptide Fragments
Potassium

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