PMID: 8588346Nov 1, 1995Paper

In vitro response of lymphocytes from bronchoalveolar lavage fluid and peripheral blood to mitogen stimulation during natural maedi-visna virus infection

Veterinary Immunology and Immunopathology
I BegaraN J Watt

Abstract

To investigate the effects of maedi-visna virus (MVV) infection on cell-mediated immunity, the in vitro response of bronchoalveolar lavage fluid (BALF) and peripheral blood (BP) lymphocytes (PBL) to exogenous mitogen was analysed. BALF and PBL from control (n = 9) and MVV-infected (n = 7) animals were cultured fro 3 days in the presence and absence of concanavalin A (Con A). Lymphocyte expression of the interleukin-2 receptor (IL-2R) antigen, a parameter of lymphocyte activation, was quantified by dual-colour flow cytometry using the bovine anti-IL-2R monoclonal antibody IL-A111. IL-2R expression by lymphocytes in BALF and PB from control and MVV-infected animals, with and without Con A stimulation, were compared. In the absence of Con A stimulation, the proportion of cultured BALF CD8+ and gamma delta T cells expressing IL-2R was significantly (P < 0.05) lower for MVV-infected animals than for controls. After Con A stimulation the proportion of BALF CD4+ lymphocytes from MVV-infected animals that expressed IL-2R remained significantly (P < 0.05) lower than for controls. Comparisons within group showed that, after Con A stimulation, the proportion of all the T cell subsets in the control group expressing IL-2R, namely CD4+ (P <...Continue Reading

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Apr 1, 1995·Veterinary Immunology and Immunopathology·I BegaraN J Watt

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Citations

Jul 27, 2001·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·J P ScharsackH J Schuberth
Sep 29, 2001·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·B SinghA de la Concha-Bermejillo
Dec 5, 1998·Journal of Comparative Pathology·R S Campbell, W F Robinson

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