PMID: 15348951Sep 7, 2004Paper

In vitro response of osteoblasts to hydroxyapatite-reinforced polyethylene composites

Journal of Materials Science. Materials in Medicine
L Di SilvioW Bonfield

Abstract

A primary human cell culture model was used to investigate a range of hydroxyapaptite (HA)-reinforced high-density polyethylene (HDPE) composites (HAPEX). These materials are being developed as potential bone-substitute materials. When designing and optimizing a second-generation biomaterial, it is important to achieve a balance between mechanical and biological properties without compromising either. Biochemical and histological parameters have been used to compare the biological response of 20% and 40% volume HA in HDPE. Cellular DNA and incorporation of tritiated thymidine was measured to assess cell proliferation. Alkaline phosphatase (ALP) production was used as a marker of osteoblast phenotype expression. In this preliminary study, osteoblasts cultured on the 20% HAPEX showed a greater increase in the rate of proliferation and osteoblast expression as indicated by an increase in ALP activity compared to the 40% HAPEX over the time period studied. Osteoblast-like cells showed a flattened morphology on both composites and in some cases a greater covering was observed on the 20% HAPEX. These results indicate that the composites may not be identical in terms of bioactivity and that further research on surface topography and p...Continue Reading

Citations

Jan 3, 2006·Journal of Materials Science. Materials in Medicine·H M T U HerathJ R G Evans
May 12, 2006·Journal of Materials Science. Materials in Medicine·A Yari SadiJ Javadpour
Mar 6, 2007·Journal of Materials Science. Materials in Medicine·Wei JieLi Yubao
Dec 25, 2007·Journal of Materials Science. Materials in Medicine·Amir Yari SadiAlireza Khavandi
Aug 9, 2002·Tissue Engineering·Matthew J DalbyWilliam Bonfield
Jul 2, 2010·Journal of the Royal Society, Interface·K E Tanner
Mar 21, 2020·Journal of Biomedical Materials Research. Part a·Xunyuan JiangYuezhong Meng

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