PMID: 6164523Jan 1, 1981Paper

In vitro RNA synthesis in oocyte nuclei of the newt Notophthalmus

Chromosoma
L D SchultzJ G Gall

Abstract

An incubation medium is described which supports RNA synthesis in isolated oocyte nuclei of the newt Notophthalmus, and which permits subsequent autoradiographic examination of the lampbrush chromosomes and nucleoli. By using different concentrations of alpha-amanitin we distinguish RNA synthesis due to RNA polymerases I, II and III. All RNA synthesis on loops is inhibited by 0.5 microgram/ml of alpha-amanitin and is therefore due to polymerase II. Polymerase III is responsible for RNA synthesis at a small number of discrete sites in condensed chromatin. These include the centromere bars of three of four chromosomes, which probably represent 5S RNA synthesis, as well as 15-20 lesser sites scattered elsewhere. Polymerase I activity is confined to the nucleoli.

References

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Citations

May 1, 1989·Chromosoma·P J DiMarioJ G Gall
Oct 1, 1986·The Journal of Cell Biology·L M EpsteinJ G Gall
Dec 1, 1989·The Journal of Cell Biology·S Piñol-RomaG Dreyfuss
Mar 1, 1992·Molecular Biology of the Cell·A TsvetkovJ G Gall
May 16, 1998·Molecular Biology of the Cell·J G Gall, C Murphy
Dec 11, 1981·Nucleic Acids Research·B K Kay, J G Gall
Nov 2, 1984·European Journal of Biochemistry·R M JohnsonJ Sommerville
May 5, 2011·Molecular Reproduction and Development·Hirohisa KyogokuJosef Fulka
Nov 13, 2009·Molecular Reproduction and Development·Hirohisa KyogokuTakashi Miyano
Aug 1, 1981·Cell·C C Hentschel, M L Birnstiel
May 12, 2000·Journal of Structural Biology·G T MorganJ G Gall
Mar 22, 1982·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·H G Callan
May 1, 2021·Pharmaceuticals·Michal BencJozef Laurincik

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