In vitro RNase and nucleic acid binding activities implicate coilin in U snRNA processing.

PloS One
Hanna J Broome, Michael D Hebert

Abstract

Coilin is known as the marker protein for Cajal bodies (CBs), subnuclear domains important for the biogenesis of small nuclear ribonucleoproteins (snRNPs) which function in pre-mRNA splicing. CBs associate non-randomly with U1 and U2 gene loci, which produce the small nuclear RNA (snRNA) component of the respective snRNP. Despite recognition as the CB marker protein, coilin is primarily nucleoplasmic, and the function of this fraction is not fully characterized. Here we show that coilin binds double stranded DNA and has RNase activity in vitro. U1 and U2 snRNAs undergo a processing event of the primary transcript prior to incorporation in the snRNP. We find that coilin displays RNase activity within the CU region of the U2 snRNA primary transcript in vitro, and that coilin knockdown results in accumulation of the 3' pre-processed U1 and U2 snRNA. These findings present new characteristics of coilin in vitro, and suggest additional functions of the protein in vivo.

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Citations

Apr 16, 2013·The International Journal of Biochemistry & Cell Biology·Michael D Hebert
Jan 16, 2013·PloS One·Valentine MakarovNatalia Kalinina
Mar 12, 2013·The Journal of Biological Chemistry·Nicole E KaySusan M D'Costa
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Sep 19, 2012·FEBS Letters·Venkatramreddy VelmaMichael D Hebert
Jan 1, 2013·Journal of Molecular Biology·Hanna J Broome, Michael D Hebert
May 15, 2015·RNA Biology·Martin MachynaDavid Staněk
Apr 16, 2015·PloS One·Isioma I EnweremMichael D Hebert
Sep 16, 2016·RNA Biology·David Staněk
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May 25, 2016·Biology Open·Aaron R Poole, Michael D Hebert
Feb 12, 2015·PloS One·Agnieszka Kołowerzo-LubnauDariusz J Smoliński
Oct 16, 2012·Chromosoma·Venkatramreddy VelmaMichael D Hebert
May 21, 2020·Molecular Biology of the Cell·Madelyn K LoganMichael D Hebert
Nov 8, 2016·RNA Biology·Michael D Hebert, Aaron R Poole
Oct 22, 2016·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Iain A SawyerMiroslav Dundr
Apr 26, 2013·Biology Open·Hanna J BroomeMichael D Hebert
Jul 29, 2021·Molecular Biology of the Cell·Katheryn E LettMichael D Hebert

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Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

Agilent MxPro Excel
MacVector
PONDR
FIT

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Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.