Abstract
The main objective of this study was to identify the antifungal metabolites from Achromobacter kerstersii JKP9, a rhizosphere bacterium isolated from tomato cultivations, inhibiting the melanin biosynthetic pathways in vascular wilt pathogen Fusarium oxysporum f. sp. lycopersici (Fol). To achieve this objective, all the rhizobacterial morphotypes were screened for plant-growth-promoting and antagonistic activities. Ethyl acetate extract of Achromobacter kerstersii JKP9 was purified in HPLC and predicted for antifungals in GC-MS equipped with Wiley library. After identification, molecular docking of useful ligands with modeled Short-chain Dehydrogenase/ Reductase (SDR) of Fol (Locus: FOXG_00472). Results were indicated that the potential strain Achromobacter kerstersii JKP9 exclusively secreted five pyrrole analogs notable for their antifungal role with no extracellular antifungal enzyme production as seen in other rhizobacterial isolates. In silico docking studies identified, Pyrrolo[1, 2-a]pyrazine-1,4-dione, hexahydro- as effective for SDR in Fol. From these results, we conclude that bacterial pyrroles can be used as an effective fungicide to control Fusarium wilt in tomatoes. In the future, these pyrrole derivatives can dire...Continue Reading
References
Oct 14, 2004·Journal of Applied Microbiology·P I KimY-T Chi
Jan 14, 2005·Microbiological Research·R DeyS M Chauhan
May 25, 2011·Journal of Industrial Microbiology & Biotechnology·Andreia Fonseca de FariaLucia Regina Durrant
May 27, 2011·The ISME Journal·Francesca MelaJohan H J Leveau
Jun 29, 2012·Chemical Biology & Drug Design·Waleed M HusseinMosaad S Mohamed
Jan 3, 2013·Microbiological Research·Vanessa Nessner KavamuraItamar Soares de Melo
Jan 18, 2015·Advances in Applied Microbiology·John A LucasBart A Fraaije
Feb 18, 2015·PloS One·An-Dong GongYu-Cai Liao
Apr 25, 2015·Journal of Microbiology and Biotechnology·Ko-Eun LeeJin-Ho Kim
Jul 5, 2016·International Journal of Systematic and Evolutionary Microbiology·Peter A VandammeJohn J LiPuma
Jul 7, 2017·Plant Physiology and Biochemistry : PPB·Vivek Kumar SinghRam Sanmukh Upadhyay
Apr 26, 2018·Frontiers in Psychology·Kate L BrookieTamlin S Conner
Nov 9, 2018·Frontiers in Pharmacology·Mohd AamirSurendra Singh
Feb 1, 2003·Plant Disease·Brett A SummerellJohn F Leslie
Apr 30, 2019·BioMed Research International·Farhana Alam RipaJian-Guang Sun
Jul 19, 2019·Frontiers in Microbiology·Dengfeng QiWei Wang
Nov 26, 2019·Saudi Journal of Biological Sciences·C SrinivasS Chandra Nayaka