In vitro selection of bacteriophage promoters employing a terminally capped template DNA and a streptavidin-binding aptamer

Journal of Bioscience and Bioengineering
Shoji Ohuchi

Abstract

In vitro selection system for the isolation of bacteriophage promoters was developed. Employing the system, one round of the selection achieved ∼20-30 fold enrichment of an active promoter from mixtures with an inactive one. In addition, active T7 promoter variants were successfully selected from a library with six randomized bases.

References

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Citations

Jul 28, 2012·Journal of Bioscience and Bioengineering·Takaaki KojimaHideo Nakano

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