In vitro selection of RNA aptamers that bind to cell adhesion receptors of Trypanosoma cruzi and inhibit cell invasion.

The Journal of Biological Chemistry
Henning UlrichWalter Colli

Abstract

Trypanosoma cruzi causing Chagas' disease needs to invade host cells to complete its life cycle. Macromolecules on host cell surfaces such as laminin, thrombospondin, heparan sulfate, and fibronectin are believed to be important in mediating parasite-host cell adhesions and in the invasion process of the host cell by the parasite. The SELEX technique (systematic evolution of ligands by exponential enrichment) was used to evolve nuclease-resistant RNA ligands (aptamer = to fit) that bind with affinities of 40-400 nm to parasite receptors for the host cell matrix molecules laminin, fibronectin, thrombospondin, and heparan sulfate. After eight consecutive rounds of in vitro selection four classes of RNA aptamers based on structural similarities were isolated and sequenced. All members of each class shared a common sequence motif and competed with the respective host cell matrix molecule that was used for displacement during the selection procedure. RNA pools following seven and eight selection rounds as well as individual aptamers sharing consensus motifs were active in inhibiting invasion of LLC-MK(2) monkey kidney cells by T. cruzi in vitro.

References

Aug 30, 1990·Nature·A D Ellington, J W Szostak
Jan 1, 1991·Experimental Parasitology·M F GonçalvesW Colli
Jan 1, 1973·Annual Review of Microbiology·Z Brener
May 1, 1982·The Journal of protozoology·N W Andrews, W Colli
Jan 1, 1993·Annual Review of Microbiology·G A Cross, G B Takle
Oct 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·W Colli
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·W PanJ F Wang
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·D O'ConnellA Varki
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·K N MorrisL Gold
Sep 15, 1998·Bioconjugate Chemistry·M C WillisB Collins
Sep 22, 1998·Antisense & Nucleic Acid Drug Development·R D JenisonD Parma
Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·H UlrichG P Hess
Apr 13, 1999·Nucleic Acids Research·M Homann, H U Göringer
Jul 30, 1999·Experimental Parasitology·M Almeida-de-FariaM J Alves
Oct 8, 1999·The Journal of Clinical Investigation·T OstendorfJ Floege
Feb 16, 2000·Journal of Biotechnology·S J HesseJ Visser
Nov 9, 2000·The Journal of Experimental Medicine·J ScharfsteinW Müller-Esterl
Nov 14, 2000·Arteriosclerosis, Thrombosis, and Vascular Biology·O LeppänenC H Heldin
Nov 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·G P HessS E Coombs
Feb 17, 2001·Biochemical and Biophysical Research Communications·S JeongJ Yu
Mar 23, 2001·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·H UlrichW Colli
Mar 30, 2001·The Journal of Biological Chemistry·M H MagdesianM J Alves
Aug 25, 2001·Experimental Biology and Medicine·I SargiannidouG P Tuszynski
Oct 11, 2001·Clinical & Experimental Metastasis·G Giannelli, S Antonaci
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·J C Rodriguez-ManzanequeM L Iruela-Arispe
Nov 1, 2001·Proceedings of the National Academy of Sciences of the United States of America·A K GhoshM Jacobs-Lorena
Dec 1, 2001·The Journal of Biological Chemistry·Ana Paula C A LimaJulio Scharfstein

❮ Previous
Next ❯

Citations

Jun 29, 2010·Applied Microbiology and Biotechnology·Hari P DwivediLee-Ann Jaykus
Dec 23, 2004·European Journal of Nuclear Medicine and Molecular Imaging·Raphaël BoisgardBertrand Tavitian
Oct 1, 2008·Histochemistry and Cell Biology·Paul Debbage, Werner Jaschke
Nov 17, 2009·In Vitro Cellular & Developmental Biology. Animal·John Gordon Bruno
Oct 7, 2003·International Journal for Parasitology·H Ulrich GöringerMihaela Lorger
Jan 16, 2008·Accounts of Chemical Research·Steven M ShamahSharon T Cload
Sep 30, 2010·Lab on a Chip·Tohid Fatanat Didar, Maryam Tabrizian
Mar 22, 2013·BioResearch Open Access·Shoji Ohuchi
Jun 6, 2009·Nucleic Acids Research·Xiaoxiao CaoNingsheng Shao
Apr 9, 2005·Nucleic Acids Research·Daniel A Di GiustoGarry C King
Jan 22, 2005·Annual Review of Medicine·Shahid M NimjeeBruce A Sullenger
Aug 19, 2007·BMC Biotechnology·Andreas NitscheAndreas Kage
May 30, 2008·Frontiers in Bioscience : a Journal and Virtual Library·Fernando VillaltaMaria F Lima
Jun 7, 2011·Therapeutic Delivery·Kristina W Thiel, Paloma H Giangrande
Dec 24, 2003·Médecine sciences : M/S·Nicolas Hugo, Philippe Bouvet
Apr 18, 2006·FEBS Letters·Kaneatra J SimmonsFernando Villalta
Sep 1, 2007·Expert Opinion on Drug Discovery·Aniela WochnerMartina Rimmele
Oct 1, 2011·Critical Reviews in Biochemistry and Molecular Biology·Jagat R KanwarRupinder K Kanwar
Feb 4, 2012·Trends in Parasitology·H Ulrich Göringer
Aug 9, 2011·Trends in Biotechnology·Weihong TanChen Liu
Jul 14, 2007·Biomolecular Engineering·Regina StoltenburgBeate Strehlitz
Jun 6, 2007·Current Opinion in Chemical Biology·Nicholas O FischerJeffrey B-H Tok
Jul 1, 2009·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Henning Ulrich, Carsten Wrenger
Dec 31, 2005·Analytical Biochemistry·Yu-Ju TsengLo-Chun Au
Nov 9, 2004·Biochemical and Biophysical Research Communications·Miryam Marroquin-QuelopanaMaria Júlia M Alves
Jun 30, 2010·Cellular & Molecular Biology Letters·Teresa Janas, Tadeusz Janas
Mar 18, 2016·Molecular Therapy : the Journal of the American Society of Gene Therapy·Sorah YoonJohn J Rossi
Jan 28, 2014·Journal of Pharmaceutical and Biomedical Analysis·Nina MencinBorut Šolar
May 2, 2013·Biotechnology Advances·Filip RadomFilip Jeleń
Sep 24, 2009·Memórias do Instituto Oswaldo Cruz·Maria Julia Manso Alves, Renato Arruda Mortara
Jul 30, 2003·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·A P M P MarinoJ Lannes-Vieira
Jul 1, 2004·Bioorganic & Medicinal Chemistry Letters·Atsushi OgawaYasuhiro Aoyama
Jul 14, 2017·Biomedicines·David E Volk, Ganesh L R Lokesh
Jun 2, 2004·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Henning UlrichJoão Bosco Pesquero

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.