In vitro signaling by MAPK and NFkappaB pathways inhibited by Yersinia YopJ.

Methods in Enzymology
Sohini Mukherjee, K Orth

Abstract

A procedure for an in vitro signaling assay is described for the MAPK and NFkappaB pathways. The method uses a membrane-cleared lysate that contains all the soluble components required for activating these signaling cascades. The pathways can be activated by variety of molecules, including kinases, G-proteins, and E3 ligases. We demonstrate that YopJ inhibits downstream of all these activators. The in vitro signaling assay is ideal for initial biochemical studies on activators and inhibitors of the MAPK and NFkappaB pathways.

Citations

Jan 12, 2013·PloS One·Francisco S MesquitaNathalie Rolhion
Apr 1, 2010·Environmental Microbiology Reports·Christian G Frank, José A Bengoechea
Nov 26, 2010·Molecular Microbiology·Sandhya V RoyanGail A Hecht
Jun 28, 2013·The Journal of Biological Chemistry·Manuel WoltersMartin Aepfelbacher
Jul 6, 2011·Molecular Plant Pathology·Jennifer D LewisDarrell Desveaux

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