In vitro spontaneous thrombin generation in human factor-IX concentrates

British Journal of Haematology
G SasJ D Cash

Abstract

A series of in vitro studies designed to ascertain the potential in vivo thrombogenicity of human factor IX-containing concentrates is described. Using concentrates obtained from several different Centres the fibrinogen clotting time with some preparations was less than 6 h and/or the recalcification time of normal plasma was shortened. In some preparations, however, the plasma recalcification time was lengthened. Further studies revealed that all diluted factor-IX concentrates generated thrombin after recalcification, and that the rate of thrombin generation appeared to be characteristic of a particular preparation. This characteristic has been designated the TGt50, which is the incubation period in minutes, after recalcification, required to obtain a 50 s clotting time of a fibrinogen substrate. The TGt50 was found to correlate most strongly with recalcification time of celite exhausted plasma (P less than 0.001), but no correlation was observed between it and the immunological antithrombin III or factor-VIII antigen levels. Evidence is presented which suggests that the thrombin generation test and recalcification time of celite exhausted plasma may represent suitable in vitro quality control assays for factor-IX concentrates.

References

Jul 19, 1973·The New England Journal of Medicine·C K Kasper
Aug 22, 1973·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·H FritschP Barth
Feb 14, 1974·The New England Journal of Medicine
Feb 14, 1974·The New England Journal of Medicine·M G Shively
Oct 1, 1971·British Journal of Haematology·P F Bruning, E A Loeliger
Oct 1, 1971·Acta Medica Scandinavica·I M NilssonG Björlin

❮ Previous
Next ❯

Citations

Nov 1, 1978·Thrombosis Research·D Menache, D L Aronson
Jan 1, 1980·Thrombosis Research·P R FosterS M Middleton
Jan 11, 2001·Thrombosis Research·D JosićA Klukowska
Jan 16, 1999·Journal of the National Cancer Institute·M HejnaC C Zielinski
Aug 1, 1977·British Journal of Haematology·D S PepperJ D Cash
Nov 1, 1977·Thrombosis Research·D S Pepper, C Prowse
Jun 15, 1989·Thrombosis Research·J ChabbatM Steinbuch
Jan 1, 1981·Annals of the New York Academy of Sciences·M H CoanM M Mozen
Jan 1, 1981·Annals of the New York Academy of Sciences·D Menache
Apr 1, 1987·British Journal of Haematology·J D LittlewoodC V Prowse
May 1, 1980·British Journal of Haematology·G W DikeC R Rizza
Nov 1, 1981·Vox Sanguinis·S Chandra, H G Brummelhuis
Jan 1, 1995·Haemophilia : the Official Journal of the World Federation of Hemophilia·D Ménaché

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.