PMID: 3319277Jan 1, 1987Paper

In vitro stability, plasma protein binding and blood cell partitioning of 14C-carboplatin

Cancer Chemotherapy and Pharmacology
R C GaverG Deeb

Abstract

Radiochemically pure 14C-labeled carboplatin, cis-diammine [1,1-cyclobutane (1-14C) dicarboxylato (2-)-0,0'] platinum (II), was added to fresh human, dog and rat plasma, at concentrations ranging from 1 to 100 micrograms 14C-carboplatin/ml. After 10 min incubation at ambient temperature, the plasma was ultrafiltered in Amicon Centrifree micropartition units to generate protein-free plasma ultrafiltrate (PU). Total radioactivity was determined by liquid scintillation counting. A mean (+/- SD) of 102% +/- 2.0%, 99.5% +/- 1.9%, and 99.0% +/- 1.0% of the 14C-carboplatin added to fresh human, dog, and rat plasma respectively was recovered in the PU. 14C-carboplatin was incubated at 37 degrees C with fresh plasma (60 micrograms/ml) and urine (200 micrograms/ml) from humans and dogs for 120 h, and samples were removed at appropriate times for analysis of carboplatin, 1,1-cyclobutane dicarboxylic acid and cyclobutane carboxylic acid. The latter were separated by HPLC on a C-18 column with a mobile phase of H2O/CH3CN/0.3 M tetrabutylammonium phosphate (880:50:20 v/v/v), and the column eluants at the retention time of each compound were collected and counted for total radioactivity. Carboplatin degraded in each of the matrices with a cor...Continue Reading

Citations

Jan 1, 1988·Cancer Chemotherapy and Pharmacology·R C GaverF M Muggia
Jan 1, 1995·Cancer Chemotherapy and Pharmacology·K ErkmenJ H Doroshow
Aug 2, 2008·Clinical and Experimental Pharmacology & Physiology·Deanna N BellMark J McKeage
Jul 1, 1993·Journal of Veterinary Internal Medicine·R L PageD E Thrall
Mar 6, 2007·Critical Reviews in Oncology/hematology·David J Stewart
Dec 4, 2010·International Journal of Cancer. Journal International Du Cancer·Paul T HendersonChong-Xian Pan
Dec 31, 2009·Biomedical Chromatography : BMC·Nicolas VillarinoTomás Martín-Jiménez
Jun 14, 2016·Environmental Science & Technology·Hangbiao JinJonathan W Martin
Feb 24, 2001·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·P E Kintzel
Jan 1, 1990·Medical and Pediatric Oncology·F DozJ M Zucker

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