PMID: 670712Jan 1, 1978Paper

In vitro stimulation of goat peripheral blood lymphocytes: optimization and kinetics of the response to mitogens and to allogeneic lymphocytes

Journal of Immunological Methods
R H van DamJ A van der Donk

Abstract

Experiments were performed to establish the optimal concentrations for specific and non-specific lymphocyte transformation in vitro, in cultures containing 10(5)-10(6) caprine lymphocytes. A microculture system was used in conjunction with a semiautomatic sample harvester. The assay was optimized for mitogen concentration (PHA-P, Con A, PWM and LPS) and allogeneic cell number, number of responding cells, incubation time and amount of tracer. The effect of addition of serum and the cytotoxic effect of phytomitogens on cultured cells is discussed.

Citations

Jan 1, 1979·Comparative Immunology, Microbiology and Infectious Diseases·J GoudswaardJ A van der Donk
Jan 1, 1996·Comparative Immunology, Microbiology and Infectious Diseases·J M MolinaO Ferrer
May 1, 1982·Veterinary Immunology and Immunopathology·E W LiewesR Bootsma
Sep 1, 1982·Veterinary Immunology and Immunopathology·K M ThamM J Studdert
Jan 1, 1982·Veterinary Immunology and Immunopathology·F KristensenS Lazáry
Aug 1, 1984·Veterinary Immunology and Immunopathology·H Kuil
Apr 1, 1985·Veterinary Immunology and Immunopathology·C E van der ZeeD Zwart
Jun 1, 1986·Veterinary Immunology and Immunopathology·R M Knott, A L Munro
Jan 1, 1988·The Veterinary Quarterly·G H WentinkP J van Kooten
Jan 1, 1979·Animal Blood Groups and Biochemical Genetics·R H van DamJ Gouswaard

Related Concepts

Metazoa
Dose-Response Relationship, Immunologic
Goat
Differential White Blood Cell Count Procedure
Lymphocyte Activation
Lymphoid Cells
Thymidine
Isolectins

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