PMID: 9450662Feb 5, 1998Paper

In vitro studies of interactions of NO. donor drugs with superoxide and hydroxyl radicals

Molecular and Cellular Biochemistry
F DallozL Rochette

Abstract

Nitric oxide (NO.) is a free radical characterized by a high spontaneous chemical reactivity with many other molecules including the superoxide radical (O2.-). This complex interaction may generate a peroxynitrite anion (ONOO-), which behaves as an important mediator of oxidative stress in many pathological states. In the present study, in vitro experiments were performed to assess directly the O2.- and hydroxyl (.OH) radical scavenging effects of various NO. donor drugs, i.e. sodium nitroprusside (SNP), sodium nitrite (NaNO2), molsidomine and SIN 1, at pH 7.4, 7 or 6. Concentrations of NO. in the incubation medium containing the different NO. donor drugs were measured by the assay based on the reaction of Fe-N-methyl-D-glucamine dithiocarbamate (MGD) with NO. that yields a stable spin-adduct measured by electron paramagnetic resonance (EPR). O2.- and .OH generation was characterized by EPR spin trapping techniques, using the spin trap 5,5-dimethyl-1-pyrroline-1-oxide (DMPO). These free radicals were generated from the enzymatic system xanthine-xanthine oxidase, in phosphate buffer adjusted at pH 7.4, 7 and 6. Under these experimental conditions, SNP exhibited the strongest superoxide scavenging properties, characterized by IC5...Continue Reading

Citations

Jul 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Frank RoseFriedrich Grimminger
Aug 23, 2002·European Journal of Clinical Investigation·J GianettiR De Caterina
Jul 12, 2005·Human & Experimental Toxicology·Sławomir KasperczykJanusz Kasperczyk
May 26, 1999·Nitric Oxide : Biology and Chemistry·G Suárez, X H Wang
Sep 1, 1999·Free Radical Biology & Medicine·K TsuchiyaR P Mason
May 2, 2006·Nitric Oxide : Biology and Chemistry·Krzysztof J ReszkaBradley E Britigan

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