In Vitro Studies on Ameliorative Effects of Limonene on Cadmium-Induced Genotoxicity in Cultured Human Peripheral Blood Lymphocytes

Applied Biochemistry and Biotechnology
Neha VermaNeeraj Aggarwal

Abstract

Cadmium (Cd) is a non-redox metal that can indirectly cause oxidative stress by depleting cellular levels of glutathione. It is well-known for the generating reactive oxygen species (ROS) such as hydroxyl radicals, superoxide anions, nitric oxide, and hydrogen peroxide. The latter inactivates antioxidant enzymes and induces lipid peroxidation and DNA damage in cells. In our study, we have investigated the ameliorative effect of limonene against Cd-induced genotoxicity using various biomarkers such as sister chromatid exchange (SCE), comet, and lipid peroxidation (LPA) assays in cultured human peripheral blood lymphocytes (PBL) from healthy individuals. It is a naturally occurring flavonoid, found in essential oil of citrus fruits. Limonene at 20-μM and 100-μM concentrations had significantly (P < 0.05 and P < 0.01) reduced the SCE frequency, tail moment, and peroxidation of lipids. Ameliorative effect of limonene was also determined by measuring the activity of antioxidant enzymes such as superoxide dismutase (SOD) and catalase (CAT). We found a significant increase (P < 0.05) in the enzyme activity after limonene treatment. We also studied the effect of GSTP1 gene polymorphism on Cd-induced genotoxicity and antigenotoxic poten...Continue Reading

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Citations

Sep 10, 2019·Human & Experimental Toxicology·K KocadalS Saygi
Dec 27, 2019·Luminescence : the Journal of Biological and Chemical Luminescence·Bin ZhangYu Zhu
Jul 1, 2020·International Journal of Molecular Sciences·Ekaterina ProshkinaAlexey Moskalev
Aug 29, 2020·The Science of the Total Environment·Yu-Mei HsuehChi-Jung Chung
Dec 9, 2020·Journal of Food Biochemistry·Pandi AnandakumarManickam Kalappan Vanitha
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lenka SochorovaJiri Sochor

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