PMID: 6968023Jan 1, 1980Paper

In vitro studies on the immunological memory for antibody response to bovine serum albumin

Microbiology and Immunology
S NishikawaY Katsura

Abstract

Immunological memory for T and B cells was studied in an in vitro culture system with spleen cells from mice primed with bovine serum albumin (BSA). Spleen cells taken from mice immunized at various times previously with a single intravenous injection of alum-precipitated (AP) BSA and bacterial endotoxin (ET) were cultured in Marbrook's system with dinitrophenylated (DNP) BSA as the in vitro antigen. In the cultures of spleen cells obtained from mice primed more than 14 days previously an IgG-predominant anti-BSA response was generated. However, no anti-BSA response was observed in the culture of spleen cells taken from mice primed 7 days previously (day 7 spleen cells). The failure of day 7 spleen cells to generate an antibody response in vitro was shown to be attributable to both the lack of B memory cells and the effect of "suppressive" macrophages induced by ET. On the other hand, anti-BSA memory in the spleen of mice primed with AP-BSA plus ET and 2 months later challenged with AP-BSA matured within 7 days and declined rather quickly by 30 days after the challenge. The difference in the time course of the generation of memory between the spleen cells from primary and from secondary immunized mice might be attributable to t...Continue Reading

References

Jan 1, 1979·Microbiology and Immunology·T Amagai, S Muramatsu
Jan 1, 1977·International Archives of Allergy and Applied Immunology·Y KatsuraI Uesaka
May 1, 1975·Cellular Immunology·T J Romano, G J Thorbecke
Nov 1, 1970·The Journal of Experimental Medicine·V Schirrmacher, K Rajewsky
Aug 1, 1974·Journal of Immunological Methods·I A Ly, R I Mishell
Dec 1, 1971·European Journal of Immunology·A J Cunningham, E E Sercarz
Nov 1, 1969·Proceedings of the Society for Experimental Biology and Medicine·M B Rittenberg, K L Pratt

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