In vitro studies on the regulation of rainbow trout (Oncorhynchus mykiss) macrophage respiratory burst activity

Developmental and Comparative Immunology
B NovoaC J Secombes

Abstract

Modulation of the respiratory burst activity of head kidney macrophages isolated from rainbow trout (Oncorhynchus mykiss) was observed following treatment with several biologically active substances. Macrophage-activating factor (MAF) induced the highest increment if respiratory burst activity relative to treatment with lipopolysaccharide (LPS), tumor necrosis factor alpha (TNF alpha) or beta-glucans from Saccharomyces cerevisiae. Increased responses were more evident when these molecules were combined in pairs. Negative regulation of respiratory burst activity was observed when diMePGE2 was added to the macrophages, with maximal inhibition seen using a concentration of 2.6 microM. Inhibition was also seen using stimulated macrophages, either by co-incubation of stimuli and diMePGE2 or by adding diMePGE2 to previously stimulated cells. The inhibitory effect on macrophages was detectable with 3 h of incubation with diMePGE2 and by 24 h the level of the response was even lower than that from unstimulated (control) macrophages. Of significance was the finding that the inhibitory effect of prostaglandin on macrophage function could be overcome by co-incubation with stimulatory molecules or by pre-treatment with MAF and LPS or MAF a...Continue Reading

References

Jan 1, 1995·Developmental and Comparative Immunology·J B Jørgensen, B Robertsen
Jan 1, 1994·Veterinary Immunology and Immunopathology·L J HardieC J Secombes
Mar 1, 1993·Developmental and Comparative Immunology·H KodamaI Azuma

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Citations

Apr 6, 2004·Developmental and Comparative Immunology·Frederick W GoetzSimon MacKenzie
Jun 5, 2004·Developmental and Comparative Immunology·Sami NikoskelainenEsa-Matti Lilius
Jan 27, 2004·Veterinary Immunology and Immunopathology·Thomas LindenstrømKurt Buchmann
Dec 13, 2000·Developmental and Comparative Immunology·F PasmansF Haesebrouck
Oct 17, 2001·Developmental and Comparative Immunology·N F NeumannM Belosevic
Jun 1, 2000·Developmental and Comparative Immunology·C Tafalla, B Novoa
Jan 28, 2003·Ecotoxicology and Environmental Safety·Iqbal AhmadM A Santos
Jun 20, 2002·Veterinary Immunology and Immunopathology·Marc Y EngelsmaB M Lidy Verburg-van Kemenade
Dec 3, 1999·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·C TafallaB Novoa
Sep 27, 2013·Fish Physiology and Biochemistry·Marcos Tucunduva de FariaJosé Roberto Machado Cunha da Silva
Oct 17, 2001·Developmental and Comparative Immunology·C J SecombesJ Zou
Jun 16, 2011·Fish & Shellfish Immunology·Cathrine A K KalgraffEirin Fausa Pettersen
Oct 16, 2015·Molecular Immunology·Victoria Gómez-Abellán, María P Sepulcre
Jun 10, 2005·Xenotransplantation·Debbie A PlouffeMiodrag Belosevic
Jun 19, 2013·Fish & Shellfish Immunology·D A Przybylska-DiazM E Nielsen
Dec 3, 2014·Fish & Shellfish Immunology·J J Miest, D Hoole
Sep 1, 2007·Fish & Shellfish Immunology·A ParamáJ Leiro
Nov 5, 2002·Clinical and Diagnostic Laboratory Immunology·L VillamilB Novoa

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