In vitro studies on the roles of transforming growth factor-beta 1 in rat metanephric development

Kidney International
A T ClarkJ F Bertram

Abstract

The development of the permanent kidney (metanephros) involves the interplay between both positive and negative regulatory molecules. Transforming growth factor-beta1 (TGF-beta 1) has previously been shown to negatively regulate ureteric duct growth. However, its potential role in nephron development and glomerulogenesis has been largely ignored. In situ hybridization and reverse transcription-polymerase chain reaction were employed to examine the temporal and spatial localization of TGF-beta 1 mRNA and a TGF-beta type I receptor (activin-like receptor kinase-5; ALK-5) mRNA in developing rat metanephroi. The addition of exogenous TGF-beta 1 to rat metanephric organ culture at different time points was used to examine the role of TGF-beta 1 in ureteric duct growth and nephron development. TGF-beta 1 mRNA did not colocalize with ALK-5 mRNA. Instead, TGF-beta1 mRNA colocalized with the TGF-beta type II receptor mRNA. The addition of recombinant human TGF-beta 1 to rat metanephric organ culture at the beginning of the culture period inhibited total metanephric growth and the growth of the ureteric tree, resulting in a decrease in nephron number. Similarly, the addition of TGF-beta 1 to metanephroi after 48 hours of culture inhibite...Continue Reading

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Citations

Aug 8, 2008·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Magdalena Kowalewska-PietrzakWojciech Mlynarski
Sep 27, 2002·Early Human Development·Jorge Reis Almeida, Carlos Alberto Mandarim-de-Lacerda
Aug 23, 2002·Trends in Cell Biology·Gregory Dressler
Oct 9, 2003·Kidney International·Kristopher SchwabS Steven Potter
Oct 29, 2009·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Almontaser HusseinFranz Schaefer
Mar 5, 2008·Pediatric Research·Sunder Sims-LucasJohn F Bertram
Sep 11, 2014·BMC Developmental Biology·Jamie A DaviesRachel Berry
Jan 14, 2017·Journal of Tissue Engineering and Regenerative Medicine·Riccardo ColettaAdrian S Woolf
Sep 27, 2005·Acta histochemica·Ipek AkilSevda Muftuoglu
Oct 7, 2005·Journal of the American Society of Nephrology : JASN·Dona T WuErwin P Böttinger
Jun 1, 2007·American Journal of Physiology. Renal Physiology·Reetu R SinghLuise A Cullen-McEwen
Aug 11, 2019·The Journal of Pathology·Filipa M LopesAdrian S Woolf
Mar 19, 2015·Molecular Reproduction and Development·Vidya K Nagalakshmi, Jing Yu
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Aug 19, 2006·Differentiation; Research in Biological Diversity·Frank Costantini
Feb 19, 2010·Differentiation; Research in Biological Diversity·Sunder Sims-LucasGeorgina Caruana

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