In vitro study in the endothelial cell compatibility and endothelialization of genipin-crosslinked biological tissues for tissue-engineered vascular scaffolds.

Journal of Materials Science. Materials in Medicine
Yu Xi-xunWan Chang-xiu

Abstract

To overcome the cytotoxicity of the chemical reagents used to fix bioprostheses, genipin, a naturally occurring crosslinking agent, was used to fix biological tissues in present study. We prepared the biological vascular scaffolds through cell extraction and fixing the porcine thoracic arteries with 1% (by w/v) genipin solution for 3 days, and then examined their mechanical properties and microstructures; glutaraldehyde- and epoxy-fixed counterparts were used as controls. HUVECs were seeded on the type I collagen-coated surface of different modified acellular vascular tissues (fixed with different crosslinking agents), and the growths of HUVECs on the specimens were demonstrated by means of MTT test, the secretion of PGI2 and vWF by HUVECs on the various specimens was also measured. Finally, HUVECs were seeded on the luminal surface of acellular biological vascular scaffolds (<6 mm internal diameter) which were, respectively, treated in the same manner described above, and then cultured for 9 days. On the ninth day, the HUVECs on the luminal surface of these vascular scaffolds were examined morphologically and by immunohistochemistry. Genipin-fixation can markedly diminish antigenicity of the vascular tissues through partially ...Continue Reading

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Citations

Jun 8, 2011·ACS Applied Materials & Interfaces·Yu-Jer HwangJulia G Lyubovitsky
Dec 26, 2012·Colloids and Surfaces. B, Biointerfaces·Hanzhou FengChunyan Zhu
Dec 22, 2015·Microvascular Research·Noriko YasudaTatsuya Shimizu
Nov 26, 2010·Journal of Biomedical Materials Research. Part a·Ting Ting LauDong-An Wang
Sep 16, 2010·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Marco C BottinoGregg M Janowski
Oct 24, 2013·Journal of Biomedical Materials Research. Part a·Kelvin L S ChanJoe Tien
Apr 27, 2013·Carbohydrate Polymers·Yuanting XuYaping Wang
Dec 4, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Amanda PinheiroSteven Elder
Jan 21, 2012·The Korean Journal of Thoracic and Cardiovascular Surgery·Jae Suk YooSeung Hwa Choi
Oct 18, 2018·Journal of Biomaterials Applications·Jianming Li, Wen Gao
Sep 2, 2019·International Journal of Biological Macromolecules·Lei XingHu-Lin Jiang

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