In vitro study of H+-sensitive neurons in the ventral medullary surface of neonate rats

Brain Research
S SugamaM Miura

Abstract

We hypothesized that the direct stimulus of the central chemoreceptor neurons is the CO2/H+-induced change in intracellular pH (pHi). If it is true, pHi responses during hypercapnic stimulation should be exhibited in the central chemoreceptor neurons in the ventral medullary surface (VMS) and some neurons in the CO2/H+ sensitive regions such as the nucleus tractus solitarii of the medial dorsal medulla (MDM). To test this hypothesis, the cultured VMS and MDM neurons (control) derived from one day-old neonate rats were labeled with H+-sensitive fluorescent indicator 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF), and were exposed to perfusate of various pHs. The H+-sensitive neurons were determined by a rapid decrease in the intracellular BCECF fluorescence intensity. In almost all the MDM neurons (99.6%) and 94% of the VMS neurons, the intracellular BCECF fluorescence intensity remained unchanged when the extracellular pH (pHo) was decreased. In contrast, in 0.4% of the MDM neurons (8/1800) and in 6% of the VMS neurons (111/1800), the intracellular BCECF fluorescence intensity decreased when the pHo was decreased from 7.4 to 7.2. This subpopulation of MDM and VMS neurons were considered to be H+-sensitive neurons. The ...Continue Reading

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Citations

Apr 19, 2005·The International Journal of Biochemistry & Cell Biology·Junichi OkadaNoriyuki Koibuchi
Mar 15, 2005·Cellular Signalling·Noriaki ShimokawaNoriyuki Koibuchi
Oct 3, 1998·Brain Research·I ArnulfM Jouvet
Jun 10, 2009·Respiratory Physiology & Neurobiology·Araya RuangkittisakulKlaus Ballanyi
Nov 5, 2004·American Journal of Physiology. Cell Physiology·Robert W PutnamNicola A Ritucci

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