In vitro study of human mutL homolog 1 hypermethylation in inducing drug resistance of esophageal carcinoma

Irish Journal of Medical Science
Y CaoG Li

Abstract

Aberrant promoter methylation of tumor suppressor gene can inhibit corresponding protein expression and promote carcinogenesis. Many studies have demonstrated that human mutL homolog 1(hMLH1) promoter methylation is correlated with occurrence and progression of multiple types of tumors. However, its correlation with esophageal carcinoma drug resistance is still unknown. To confirm methylation status of hMLH1 promoter in drug-resistance cell line of esophageal carcinoma, further confirm whether hMLH1 promoter methylation is responsible for drug resistance. Two stable esophageal carcinoma drug-resistance cell lines were successfully established by Cisplatin (DDP) concentration increment method; methylation status of hMLH1 promoter, mRNA and protein expression of hMLH1 were detected by methylation-specific PCR (MSP), RT-PCR and western blot, respectively; Drug-resistance ability assay was used to detect drug-resistance ability. Stronger methylation status of hMLH1 promoter, lower hMLH1 mRNA and protein expression were found in both drug-resistance cell lines; after removing methylated bands using 5-aza-2'-deoxycytidine(5-Aza-CdR) in drug-resistance cell lines, hMLH1 mRNA and protein expression were restored and drug-resistance abi...Continue Reading

References

Jun 28, 2001·The Journal of Biological Chemistry·C SchmutteR Fishel
Dec 6, 2003·The New England Journal of Medicine·Peter C Enzinger, Robert J Mayer
Dec 8, 2005·Carcinogenesis·Vincent O'Brien, Robert Brown
Jun 5, 2008·Nucleic Acids Research·Harma FeitsmaEdwin Cuppen

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Citations

Jan 3, 2020·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Sunao FujiyoshiAkinobu Taketomi

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