In vitro study of novel gadolinium-loaded liposomes guided by GBI-10 aptamer for promising tumor targeting and tumor diagnosis by magnetic resonance imaging

International Journal of Nanomedicine
Meng-Jie GuTian-Yuan Fan

Abstract

Novel gadolinium-loaded liposomes guided by GBI-10 aptamer were developed and evaluated in vitro to enhance magnetic resonance imaging (MRI) diagnosis of tumor. Nontargeted gadolinium-loaded liposomes were achieved by incorporating amphipathic material, Gd (III) [N,N-bis-stearylamidomethyl-N'-amidomethyl] diethylenetriamine tetraacetic acid, into the liposome membrane using lipid film hydration method. GBI-10, as the targeting ligand, was then conjugated onto the liposome surface to get GBI-10-targeted gadolinium-loaded liposomes (GTLs). Both nontargeted gadolinium-loaded liposomes and GTLs displayed good dispersion stability, optimal size, and zeta potential for tumor targeting, as well as favorable imaging properties with enhanced relaxivity compared with a commercial MRI contrast agent (CA), gadopentetate dimeglumine. The use of GBI-10 aptamer in this liposomal system was intended to result in increased accumulation of gadolinium at the periphery of C6 glioma cells, where the targeting extracellular matrix protein tenascin-C is overexpressed. Increased cellular binding of GTLs to C6 cells was confirmed by confocal microscopy, flow cytometry, and MRI, demonstrating the promise of this novel delivery system as a carrier of MRI...Continue Reading

Citations

Jan 26, 2017·Journal of the American Chemical Society·Liqin ZhangWeihong Tan
Mar 11, 2020·Journal of Cellular and Molecular Medicine·Andrés Rodríguez-GalvánVladimir A Basiuk
Nov 23, 2016·Journal of Cell Science·Kim S MidwoodGertraud Orend
Mar 18, 2021·Journal of Liposome Research·Hanieh AbbasiSomayeh Handali

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Methods Mentioned

BETA
SELEX
dynamic light scattering
fluorescence microscopy
flow cytometry
FCS
flow
confocal microscopy
confocal
chemical modification

Software Mentioned

FCS Express

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