PMID: 6162900Apr 1, 1981Paper

In vitro study of the binding of antiribonucleoprotein antibodies to the nucleus of isolated living keratinocytes

The Journal of Investigative Dermatology
L Galoppin, J H Saurat

Abstract

The general accepted dogma is that antibodies do not penetrate living cells. However, this has recently been challenged for anti-ribonucleoprotein antibodies (anti-RNPab). We have studied here the "penetration" of trypsin isolated keratinocytes in vitro, using indirect immuno-fluorescence and immuno-peroxidase techniques. 70% (+/- 22) of a living keratinocyte cell suspension showed nuclear speckled staining when incubated with anti-RNP sera but only 9.5% (+/- 4) were stained with an anti-DNA antiserum (homogeneous pattern). A suspension of dead keratinocytes gave similar percentages with both anti-sera (89.5 [+/- 8] and 76.6 [+/- 18] respectively). The penetration of anti-RNPab into the nuclei of living epidermal cells increased gradually during the first hour of incubation without a parallel increase in the deadh rate measured by trypan blue dye exclusion. There was still high percentage of stained cell even after high dilution (1/1000) of anti-RNP sera. However, the percentage markedly decreased after previous incubation of the cells with increasing concentrations of concanavalin A. No decrease was obtained with dead keratinocytes in the same conditions. After they had been incubated with anti-RNPab, the epidermal cells were ...Continue Reading

Citations

Jul 1, 1991·The British Journal of Dermatology·M S KoelleT P Chorzelski
May 1, 1985·The British Journal of Dermatology·A ParodiA Rebora
Dec 1, 1983·The Journal of Dermatology·Y KobayashiS Sano
Sep 1, 1983·The British Journal of Dermatology·K Iwatsuki
Oct 7, 2004·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Paul BerghuisTibor Harkany
Mar 25, 2016·Nature Reviews. Rheumatology·Philip W NobleJames E Hansen
Jan 1, 1985·The British Journal of Dermatology·Z Y ChenH R Maricq
May 23, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·R H WeisbartR Nishimura
Sep 26, 2007·Annals of the New York Academy of Sciences·Margherita SistoVincenzo Mitolo
Dec 2, 2008·Clinical and Experimental Dermatology·J X SousaV Aoki
Apr 1, 1996·Immunology Today·D Alarcon-SegoviaL Llorente
Mar 1, 1993·The Journal of Investigative Dermatology·T D GolanK B Elkon

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