In vitro study on interaction between human nucleus pulposus cells and mesenchymal stem cells through paracrine stimulation

Spine
Shu-Hua YangFeng-Huei Lin

Abstract

Coculture of human nucleus pulposus (NP) cells and mesenchymal stem cells (MSCs) using a noncontact system. To investigate the interaction between NP cells and MSCs through paracrine stimulation. Cell-based therapies have a potential role in the treatment of intervertebral disc degeneration. Upregulating the viability of NP cells and differentiating MSCs into NP-like cells are potential alternatives to achieve viable cells. Culture plates and inserts were used to coculture MSCs and NP cells without direct contact or exchange of cellular components. Cellular proliferation and RNA expression of selected genes were then evaluated after coculture. Coculturing slightly promoted the proliferation of MSCs, and expression of collagen I and Fas-associated death domain protein significantly decreased. MSCs, which initially expressed no collagen II, started to show collagen II expression after coculturing; the expression level was highest when the cells were cultured with a higher number of NP cells. On the converse, proliferation of NP cells significantly rose even after cocultured with a few MSCs. Increasing the number of cocultured MSCs did not further enhance proliferation of NP cells. Expression of aggrecan in the NP cells significan...Continue Reading

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Citations

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