In-vitro subsurface remineralisation of artificial enamel white spot lesions pre-treated with chitosan

Dental Materials : Official Publication of the Academy of Dental Materials
Jing ZhangAvijit Banerjee

Abstract

To test the null hypothesis that chitosan application has no impact on the remineralisation of artificial incipient enamel white spot lesions (WSLs). 66 artificial enamel WSLs were assigned to 6 experimental groups (n=11): (1) bioactive glass slurry, (2) bioactive glass containing polyacrylic acid (BG+PAA) slurry, (3) chitosan pre-treated WSLs with BG slurry (CS-BG), (4) chitosan pre-treated WSLs with BG+PAA slurry (CS-BG+PAA), (5) remineralisation solution (RS) and (6) de-ionised water (negative control, NC). Surface and cross-sectional Raman intensity mapping (960cm-1) were performed on 5 samples/group to assess mineral content. Raman spectroscopy and attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) were used to identify the type of newly formed minerals. Surface and cross-sectional Knoop microhardness were implemented to evaluate the mechanical properties after remineralisation. Surface morphologies and Ca/P ratio were observed using scanning electron microscopy (SEM) coupled with energy dispersive X-ray spectroscopy (EDX). Data were statistically analysed using one-way ANOVA with Tukey's test. BG+PAA, CS-BG, RS presented significantly higher mineral regain compared to NC on lesion surfaces, wh...Continue Reading

Citations

Dec 18, 2019·Materials·Lin Lu DaiEdward Chin Man Lo
Jul 1, 2020·International Journal of Biological Macromolecules·Elaheh FakhriHossein Samadi Kafil
Aug 18, 2020·Dental Materials : Official Publication of the Academy of Dental Materials·Wen ZhouHockin H K Xu
Jul 11, 2021·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ramya RamadossBalakumar Subramanian
Jul 2, 2020·Dental Materials : Official Publication of the Academy of Dental Materials·Caroline MocquotPierre Colon

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