In vitro sugar uptake by grapefruit (Citrus paradisi) juice-sac cells

Functional Plant Biology : FPB
Moshe HubermanRaphael Goren

Abstract

To further our understanding of the mechanisms of sugar uptake and accumulation into grapefruit (Citrus paradisi Macf. cv. Marsh seedless), the patterns of uptake and utilisation of sucrose, glucose and fructose by Citrus juice cells was investigated. Analyses were conducted on sliced juice sacs that were incubated in radioactive [14C]-sugar solutions with unlabelled sugars, in the presence or absence of metabolic inhibitors. Both hexoses demonstrated an initial uptake peak in December and a second uptake peak in February-March. From March through April the rates of sucrose uptake increased to levels comparable to those of glucose and fructose. Sucrose and its moieties fructose and glucose entered the juice cells of Citrus juice fruit by an insaturable, and mostly by an independent, process. However, NaN3 and carbonylcyanide m-chlorophenylhydrazone (CCCP) produced slight inhibition of these processes. Cells took up hexoses at a greater rate than sucrose, with accumulation reaching a plateau by 4-8 h, and then continuing unabated, in the case of glucose, for 42 h. Uptake of all three sugars increased linearly in the range of sugar concentrations tested, which extended from 0.01 to 320 mm, denoting an insaturable system for sugar...Continue Reading

References

Mar 15, 1974·Biochimica Et Biophysica Acta·D M Regen, H L Tarpley
Apr 5, 2000·Biochimica Et Biophysica Acta·R Lemoine
Nov 1, 1979·Plant Physiology·R Wyse
Nov 1, 1980·Plant Physiology·R A Saftner, R E Wyse
Jul 1, 1982·Plant Physiology·J Riov, S F Yang
May 1, 1983·Plant Physiology·R A SaftnerR E Wyse
Jun 1, 1983·Plant Physiology·R T GiaquintaV R Franceschi
Jun 1, 1986·Plant Physiology·R E WyseA D Tomos
Jul 1, 1988·Plant Physiology·S DamonA B Bennett
Jun 1, 1989·Plant Physiology·E Echeverria, J K Burns
Jul 1, 2003·Functional Plant Biology : FPB·Sarah PicaudSerge Delrot
Jun 1, 2002·Functional Plant Biology : FPB·Shelley R McRaeJoseph A M Holtum

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Methods Mentioned

BETA
scintillation spectroscopy

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