In vitro survival of follicles in prepubertal ewe ovarian cortex cryopreserved by slow freezing or non-equilibrium vitrification

Journal of Assisted Reproduction and Genetics
Yann LocatelliMichael J Bertoldo

Abstract

Vitrification is a well-accepted fertility preservation procedure for cryopreservation of oocytes and embryos but little is known regarding ovarian tissue, for which slow freezing is the current convention. The aim of the present study was to assess the efficiency of non-equilibrium vitrification compared to conventional slow freezing for ovarian cortex cryopreservation. Using prepubertal sheep ovaries, the capacity of the tissue to sustain folliculogenesis following cryopreservation and in vitro culture was evaluated. Ovarian cortex fragments were cultured in wells for 9 days, immediately or after cryopreservation by conventional slow freezing or non-equilibrium vitrification in straws. During culture, follicular populations within cortex were evaluated by histology and immunohistochemistry for PCNA and TUNEL. Steroidogenic activity of the tissue was monitored by assay for progesterone and estradiol in spent media. No significant differences in follicle morphology, PCNA, or TUNEL labeling were observed between cryopreservation methods at the initiation of culture. Similar decreases in the proportion of primordial follicle population, and increases in the proportion of growing follicles, were observed following culture of fresh...Continue Reading

References

Nov 18, 1997·Human Reproduction·S A WandjiJ E Fortune
Jun 5, 2003·European Journal of Obstetrics, Gynecology, and Reproductive Biology·E IsachenkoF Nawroth
Oct 13, 2007·Cryobiology·Vladimir IsachenkoHans van der Ven
Feb 28, 2009·The New England Journal of Medicine·Jacqueline S Jeruss, Teresa K Woodruff
Mar 7, 2009·Biology of Reproduction·Candace M TingenTeresa K Woodruff
Apr 11, 2009·Human Reproduction·Victoria KerosOuti Hovatta
Apr 30, 2009·Reproductive Biomedicine Online·Noriko KagawaMasashige Kuwayama
May 15, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·V IsachenkoJ M Weiss
Jul 15, 2009·Human Reproduction·Min XuTeresa K Woodruff
Jan 30, 2010·PloS One·W Hamish B Wallace, Thomas W Kelsey
Apr 20, 2011·The Journal of Obstetrics and Gynaecology Research·Hye Jin ChangSeok Hyun Kim
May 18, 2011·Clinical Endocrinology·Richard A Anderson, W Hamish B Wallace
Jun 10, 2011·Reproductive Biology and Endocrinology : RB&E·Ghaya MerdassiJacqueline Lornage
Jun 17, 2011·Reproductive Biomedicine Online·Christiani A AmorimJacques Donnez
May 9, 2012·Clinical and Experimental Reproductive Medicine·Jin Yeong Kim
Aug 14, 2012·Fertility and Sterility·Christiani Andrade AmorimAnne Van Langendonckt
Oct 30, 2013·Nature Reviews. Endocrinology·Jacques Donnez, Marie-Madeleine Dolmans
Aug 3, 2014·Animal Reproduction Science·Michael J BertoldoYann Locatelli
Oct 7, 2014·Lancet·Michel De VosTeresa K Woodruff
Dec 9, 2014·Reproduction, Fertility, and Development·Luciana R FaustinoAna Paula R Rodrigues
Jul 27, 2015·Journal of Assisted Reproduction and Genetics·Jacques Donnez, Marie-Madeleine Dolmans
Feb 26, 2016·Molecular Human Reproduction·Michael J BertoldoYann Locatelli
Nov 18, 2016·The Journal of Endocrinology·Ebrahim AsadiFarid Abolhassani
Nov 4, 2017·Journal of Assisted Reproduction and Genetics·Zohar GavishDror Meirow
Jan 16, 2018·Reproduction, Fertility, and Development·N J DonfackA P R Rodrigues
Mar 6, 2018·Reproductive Biomedicine Online·Michael J BertoldoYann Locatelli
Sep 12, 2018·Reproduction in Domestic Animals = Zuchthygiene·Nathalie Jiatsa DonfackAna Paula Ribeiro Rodrigues

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.