In vitro swelling of the human sperm nucleus in the presence of sodium dodecyl sulphate

Archives of Andrology
S JagerJ Kremer

Abstract

The in vitro swelling of human sperm nuclei in the presence of sodium dodecyl sulphate (SDS) was studied by counting swollen sperm nuclei in a microscopic preparation and by measuring the decrease of optical density at 600 nm (OD600). At pH 8.0 or lower, OD600 showed a linear relationship to the sperm count. At higher pH values a fraction of the nuclei became swollen and OD600 decreased proportionally, although the sperm count did not change in the first 2 hr. The fraction of swollen sperm nuclei increased with time and by raising the pH or temperature. The swelling occurred only in the presence of SDS and was the consequence of a binding of SDS to the hydrophobic regions of the nuclear proteins.

References

Mar 1, 1966·Experimental Cell Research·B L GledhillN R Ringertz
Jul 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·J A Reynolds, C Tanford
Mar 25, 1956·The Journal of Biophysical and Biochemical Cytology·M ALFERT

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Citations

Jun 16, 2005·Theriogenology·Ninoska Madrid-BuryJulio de la Fuente Martínez
Jan 1, 1986·Archives of Andrology·J L Huret
Jan 1, 1986·Archives of Andrology·D Le LannouJ Segalen

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