In vitro synergistic effect of amphotericin B and allicin on Leishmania donovani and L. infantum

Antimicrobial Agents and Chemotherapy
María Jesús CorralJ M Alunda

Abstract

Current monotherapy against visceral leishmaniasis has serious side effects, and resistant Leishmania strains have been identified. Amphotericin B (AmB) has shown an extraordinary antileishmanial efficacy without emergence of resistance; however, toxicity has limited its general use. Results obtained showed, using a fixed-ratio analysis, that the combination of diallyl thiosulfinate (allicin) and AmB ranged from moderately synergic to synergic at low concentrations (0.07 μM AmB plus 35.45 μM allicin induced 95% growth inhibition). None of the treatments, alone or in combination, had noticeable adverse effects on macrophages (M) in the concentration range examined (allicin, 0.5, 1, 5 and 10 μM; AmB, 0.05, 0.075, and 0.1 μM). Allicin, AmB, or the combination did not affect the infection rate (percentage of infected M) of Leishmania. Allicin enhanced the activity of AmB on intracellular amastigotes of Leishmania donovani and L. infantum (ca. 45% reduction of amastigote burden with 0.05 μM AmB plus 10 μM allicin); this represented nearly a 2-fold reduction in the 50% inhibitory concentration (IC50) of the antibiotic added alone. Results point toward the possible utility of testing this combination in vivo to reduce the toxicity ass...Continue Reading

Citations

Aug 7, 2014·The Journal of Antimicrobial Chemotherapy·M Jesús CorralJosé M Alunda
Jul 18, 2015·Parasitology Research·Neeradi DineshSushma Singh
May 6, 2015·Drug Discovery Today·Rajalakshmi Rajasekaran, Yi-Ping Phoebe Chen
Aug 13, 2014·Antimicrobial Agents and Chemotherapy·Christian FerreiraElvira Maria Saraiva
Dec 12, 2018·Journal of Biomolecular Structure & Dynamics·Haraprasad MandalPradeep Das

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