In vitro synthesis of 9,10-dihydroxyhexadecanoic acid using recombinant Escherichia coli

Microbial Cell Factories
Anees KaprakkadenVirendra Swarup Bisaria

Abstract

Hydroxy fatty acids are widely used in food, chemical and cosmetic industries. A variety of dihydroxy fatty acids have been synthesized so far; however, no studies have been done on the synthesis of 9,10-dihydroxyhexadecanoic acid. In the present study recombinant E. coli has been used for the heterologous expression of fatty acid hydroxylating enzymes and the whole cell lysate of the induced culture was used for in vitro production of 9,10-dihydroxyhexadecanoic acid. A first of its kind proof of principle has been successfully demonstrated for the production of 9,10-dihydroxyhexadecanoic acid using three different enzymes viz. fatty acid desaturase (FAD) from Saccharomyces cerevisiae, epoxide hydrolase (EH) from Caenorhabditis elegance and epoxygenase (EPOX) from Stokasia laevis. The genes for these proteins were codon-optimised, synthesised and cloned in pET 28a (+) vector. The culture conditions for induction of these three proteins in E. coli were optimised in shake flask. The induced cell lysates were used both singly and in combination along with the trans-supply of hexadecanoic acid and 9-hexadecenoic acid, followed by product profiling by GC-MS. Formation of 9,10-dihydroxyhexadecanoic acid was successfully achieved when...Continue Reading

References

Aug 14, 1999·The Journal of Biological Chemistry·A R Brash
May 1, 1965·The Biochemical Journal·A T JAMESJ P WEBB
Apr 2, 2005·Biotechnology Progress·Simona JevsevarViktor Menart
Apr 11, 2006·Applied Microbiology and Biotechnology·J O Metzger, U Bornscheuer
Nov 23, 2007·The Biochemical Journal·Imke LangIvo Feussner
Aug 31, 2010·Archives of Biochemistry and Biophysics·Igor IvanovMatthias Walther
Dec 15, 2010·Applied Microbiology and Biotechnology·Min-Jung SuhHak-Ryul Kim
Dec 16, 2010·The FEBS Journal·Inge N A Van BogaertWim Soetaert
Jun 17, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Daniel Arrieta-BaezL Gerardo Zepeda-Vallejo
Mar 1, 2012·Applied Microbiology and Biotechnology·Roman HolicHiroshi Uemura
Jul 19, 2013·Biotechnology Advances·Kyoung-Rok Kim, Deok-Kun Oh
Apr 19, 2015·Microbial Cell Factories·Anupam SinghAmulya Kumar Panda

❮ Previous
Next ❯

Citations

May 23, 2018·Applied Microbiology and Biotechnology·Matthias Engleder, Harald Pichler

❮ Previous
Next ❯

Datasets Mentioned

BETA
ABV45408
AAR23815

Software Mentioned

GenScript OptimumGene
GenScript

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.