In vitro synthesis of high molecular weight rubber by Hevea small rubber particles

Journal of Bioscience and Bioengineering
Porntip RojruthaiYasuyuki Tanaka

Abstract

Hevea brasiliensis is one of few higher plants producing the commercial natural rubber used in many significant applications. The biosynthesis of high molecular weight rubber molecules by the higher plants has not been clarified yet. Here, the in vitro rubber biosynthesis was performed by using enzymatically active small rubber particles (SRP) from Hevea. The mechanism of the in vitro rubber synthesis was investigated by the molecular weight distribution (MWD). The highly purified SRP prepared by gel filtration and centrifugation in the presence of Triton((R)) X-100 showed the low isopentenyl diphosphate (IPP) incorporation for the chain extension mechanism of pre-existing rubber. The MWD of in vitro rubber elongated from the pre-existing rubber chains in SRP was analyzed for the first time in the case of H. brasiliensis by incubating without the addition of any initiator. The rubber transferase activity of 70% incorporation of the added IPP (w/w) was obtained when farnesyl diphosphate was present as the allylic diphosphate initiator. The in vitro synthesized rubber showed a typical bimodal MWD of high and low molecular weight fractions in GPC analysis, which was similar to that of the in vivo rubber with peaks at around 10(6) ...Continue Reading

Citations

Sep 17, 2013·Biochimica Et Biophysica Acta·Karine BerthelotFrédéric Peruch
Apr 2, 2020·Annual Review of Biochemistry·Satoshi Yamashita, Seiji Takahashi
May 30, 2019·International Journal of Molecular Sciences·Quanliang XieXuchu Wang

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