Abstract
A ribosomal fraction of Trypanosoma cruzi was isolated with detergent lysis and differential ultracentrifugation. This ribosomal fraction directed in vitro protein synthesis, in a heterologous incorporation system (rat liver pH 5 fraction), leading to values up to 10 times higher than endogenous control. The ideal concentrations of Mg2+, K+, and ribosomal proteins and the time of incubation of the incorporation mixtures were 6 mM, 21 mM, 60 microliters, and 45 min, respectively. The product thus obtained was analyzed by fluorography after polyacrylamide-sodium dodecyl sulfate gel electrophoresis and showed the presence of many protein bands, but few bands were present in the immunoprecipitate obtained with serum from Chagas' disease patients. This product was able to react with anti-T. cruzi antibodies when submitted to an immunoenzymatic assay.
References
Oct 10, 1979·Nucleic Acids Research·M LheureuxM Steinert
Feb 1, 1978·The Journal of protozoology·N M Morales, J F Roberts
Sep 1, 1978·Nucleic Acids Research·R O WilliamsS C Williams
Oct 1, 1977·Parasitology·M J EggittK N Brown
Oct 18, 1976·Biochemical and Biophysical Research Communications·A M StolfR Brentani
Feb 15, 1968·Biochemical and Biophysical Research Communications·N TanakaH Masukawa
Aug 21, 1973·Biochemical and Biophysical Research Communications·M T Huang, A P Grollman
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Feb 1, 1967·Biochemistry·I H Goldberg, K Mitsugi
May 17, 1969·Nature·Y OnoP Lengyel
Jan 1, 1969·Immunochemistry·S Avrameas
Jul 1, 1981·Archives of Biochemistry and Biophysics·E RondinelliF T de Castro
Aug 1, 1980·The Journal of protozoology·M S Crane, J A Dvorak
Feb 1, 1981·Molecular and Biochemical Parasitology·C CastroM Castañeda
Jun 1, 1980·Molecular and Biochemical Parasitology·S C Merritt