PMID: 1201873Dec 15, 1975Paper

In vitro synthesis of tumor-specific factors with blocking and antibody-dependent cellular cytotoxicity (ADC) activities

International Journal of Cancer. Journal International Du Cancer
K NelsonK E Hellström

Abstract

Spleen cells from BALB/c mice bearing syngeneic sarcomas and from BALB/c mice whose sarcomas had been excised were cultivated in vitro. The culture supernatants were tested for two activities: their ability (1) to supress ("block") specific lymph-node cell-mediated cytotoxic reactions directed against the respective neoplasms; and (2) to induce antibody-dependent cellular cytotoxicity (ADC) specific for the antigens of the respective tumors. Both specific activities, blocking and induction of ADC, were detected in culture supernatants from spleens of tumor-bearing mice, even when repeatedly harvested at intervals over a 7-day period. Supernatants of cultured spleen cells from mice whose sarcomas had been excised 3-4 weeks previously also had ADC but no blocking activity. Supernatnats of cultures treated with inhibitors of protein synthesis lacked both blocking and ADC activities; the inhibitory effect of cycloheximide on these activities, as well as on protein synthesis, was reversible. Factors in the culture supernatants responsible for blocking and for ADC were labelled when the culture were incubated with 14C-leucine. The labelled material was retained by, and could be eluted from, immunoadsorbents for mouse immunoglobulins....Continue Reading

References

Sep 15, 1975·International Journal of Cancer. Journal International Du Cancer·J TameriusK E Hellström
Nov 15, 1975·International Journal of Cancer. Journal International Du Cancer·I HellströmJ T Nepom
May 1, 1973·International Journal of Cancer. Journal International Du Cancer·R W BaldwinR A Robins
Sep 1, 1974·The Journal of Experimental Medicine·C S HenneyB R Bloom
Nov 15, 1974·International Journal of Cancer. Journal International Du Cancer·R A Robins, R W Baldwin
Mar 15, 1972·International Journal of Cancer. Journal International Du Cancer·S PollackK Nelson
Jun 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·H O SjögrenK E Hellström
Jul 1, 1968·Immunochemistry·W B Dandliker, V A De Saussure

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Citations

Mar 15, 1983·Experientia·G T NepomK E Hellström
Apr 1, 1988·Immunology and Cell Biology·J M Greer, W J Halliday
Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·K E Hellström, I Hellström
Feb 15, 1983·Cellular Immunology·T A Koppi, W J Halliday
Mar 1, 1981·Medical Hypotheses·R Warlow
Jan 1, 1976·Annals of the New York Academy of Sciences·K E Hellström, I Hellström

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