PMID: 6163570Feb 9, 1981Paper

In vitro synthesis of tyrosine hydroxylase

Comptes rendus des séances de l'Académie des sciences. Série III, Sciences de la vie
J MalletJ F Pujol

Abstract

RNA from PC 12 cells was translated in a cell-free system and the peptides synthesized in vitro were analyzed by immunoprecipitation with an anti-T.H. serum raised in Rabbit. Analysis of the immunoprecipitate on a SDS-polyacrylamide gel reveals a major band with a molecular weight of 62000 dalton. A protein of the same molecular weight was also precipitated from a PC12 cell lysate. This molecular weight of 62000 dalton corresponds to the reported for tyrosine hydroxylase purified from PC12 cells.

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