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In vitro synthesis of uniform poly(dG)-poly(dC) by Klenow exo- fragment of polymerase I

Nucleic Acids Research

Jan 28, 2005

Alexander KotlyarMichael Gozin

Abstract

In this paper, we describe a production procedure of the one-to-one double helical complex of poly(dG)-poly(dC), characterized by a well-defined length (up to 10 kb) and narrow size distribution of molecules. Direct evidence of strands slippage during poly(dG)-poly(dC) synthesis by Klen...read more

Mentioned in this Paper

Polymerase
poly(dG).poly(dC)
Transcription Initiation
DNA Polymerase I
Fluorescent stain
Mass Spectrometry
Oligonucleotide Primers
Nucleotides
DNA Replication
Dyes [MoA]
Paper Details
References
  • References26
  • Citations21
123
  • References26
  • Citations21
123

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In vitro synthesis of uniform poly(dG)-poly(dC) by Klenow exo- fragment of polymerase I

Nucleic Acids Research

Jan 28, 2005

Alexander KotlyarMichael Gozin

PMID: 15673713

DOI: 10.1093/nar/gki178

Abstract

In this paper, we describe a production procedure of the one-to-one double helical complex of poly(dG)-poly(dC), characterized by a well-defined length (up to 10 kb) and narrow size distribution of molecules. Direct evidence of strands slippage during poly(dG)-poly(dC) synthesis by Klen...read more

Mentioned in this Paper

Polymerase
poly(dG).poly(dC)
Transcription Initiation
DNA Polymerase I
Fluorescent stain
Mass Spectrometry
Oligonucleotide Primers
Nucleotides
DNA Replication
Dyes [MoA]

Feeds With Similar Papers

Non-coding Variants in Cancer (Keystone)

Mutations, single nucleotide variants and large genomic rearrangements in non-coding regions of the genome can have severe consequences such as disruption of transcription factor binding sites and functions of non-coding RNAs and can impact tumor development and progression. This feed focuses on the latest research on non-coding variants in cancer.

Non-coding Variants in Cancer

Mutations, single nucleotide variants and large genomic rearrangements, in non-coding regions of the genome can have severe consequences such as disruption of transcription factor binding sites and functions of non-coding RNAs and can impact tumor development and progression. Here is the latest research.

Related Papers

Paper Details
References
  • References26
  • Citations21
123
  • References26
  • Citations21
123

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