PMID: 6384730Jan 1, 1984

In vitro template activity of 0.3 mRNA from wild type and initiation mutants of bacteriophage T7

Molecular & General Genetics : MGG
H OhsawaC O Gualerzi


Bacteriophage T7 0.3 mRNA synthesised and processed in vitro has been purified starting from the DNA of T7+ as well as from that of two initiation mutants of T7 (CR17 with a U----C transition in the initiation codon and CR35b whose potential Shine and Dalgarno (S-D) interaction is interrupted by a G----A transition). These mRNAs were used as templates to direct the binding of fMet-tRNA and the synthesis of 0.3 protein in both E. coli and wheat germ cell-free systems. The initiation codon mutant displayed approximately 50% inhibition of fMet-tRNA binding and 0.3 protein synthesis in both systems. The S-D sequence mutant, on the other hand, was found to be less affected than the initiation triplet mutant (20%-40% inhibition) in both fMet-tRNA binding and template activity in the E. coli system. In the wheat germ system, which does not make use of the S-D interaction, however, this mutant displayed normal template activity suggesting that the inhibition obtained in the E. coli system, albeit slight, is due to the impairment of the S-D interaction and not to an alteration of the mRNA secondary or tertiary structure caused by the base substitution.


Oct 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·P Herrlich, M Schweiger
Dec 25, 1979·Journal of Molecular Biology·F W StudierJ J Dunn
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J J DunnF W Studier
Dec 15, 1975·Journal of Molecular Biology·J J Dunn, F W Studier
Aug 25, 1977·Journal of Molecular Biology·J A Steitz, R A Bryan
Jan 1, 1973·Annual Review of Genetics·G Zubay
Mar 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·K HerculesW Sauerbier
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J Shine, L Dalgarno
Sep 15, 1973·Journal of Molecular Biology·F W Studier
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Jan 1, 1981·Annual Review of Microbiology·L GoldG Stormo
Feb 5, 1980·Journal of Molecular Biology·S Strome, E T Young
May 11, 1982·Nucleic Acids Research·G StormoL Gold
Jun 5, 1981·Journal of Molecular Biology·J J Dunn, F W Studier
Dec 1, 1958·Biochimica Et Biophysica Acta·R F HILL


Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R A CalogeroC O Gualerzi
Aug 12, 2015·Cellular and Molecular Life Sciences : CMLS·C O Gualerzi, Cynthia L Pon

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