In-vitro testicular bioactivation of acrylonitrile

Pharmacological Research : the Official Journal of the Italian Pharmacological Society
A H Abdel-AzizA E Ahmed

Abstract

The present work examines the mechanism of testicular toxicity of acrylonitrile. In testicular centrifugal fractions from Sprague Dawley rats, the metabolism of VCN to cyanide (CN-) was highest in the microsomal fraction and required NADPH for maximum activity. This biotransformation of VCN to CN- was characterized with respect to time (30 min), microsomal protein concentration (1.5 mg ml(-1)), pH (7.5) and temperature (37 degrees C). The V(max) of the reaction was 65.1 pmol CN- mg protein(-1) min(-1) and K(m) was 88.6 micromol VCN. Flushing the microsomes with carbon monoxide (CO)(4:1, CO/O2 v/v), addition of benzimidazole (1 mM) or addition of SKF 525-A (5x10(-4) M) to incubation mixtures significantly inhibited VCN metabolism by 49%, 54% and 37.4% respectively. Activation of VCN to CN- was markedly increased in microsomes obtained from phenobarbital (PB)-treated rats (128.2%). Addition of glutathione (GSH), L-cysteine, D-penicillamine or 2-mercaptoethanol significantly enhanced the release of CN- from VCN 126%, 247%, 202% and 129% of the control value respectively. These findings indicate that VCN is metabolized in the testis via cytochrome P-450 dependent mixed function oxidase system.

References

Dec 1, 1992·Bulletin of Environmental Contamination and Toxicology·D CovaA Santagostino
Sep 1, 1992·Trends in Pharmacological Sciences·F J Gonzalez
Jan 1, 1992·Journal of Biochemical Toxicology·A E AhmedA M Nour-al Deen
Apr 1, 1992·Scandinavian Journal of Work, Environment & Health·L G SolionovaJ V Plotnikov
Sep 1, 1990·Biomaterials·S Parker, M Braden
Jan 1, 1990·Journal of Biochemical Toxicology·M Y FarooquiR Cavazos
Nov 1, 1989·Chemical Research in Toxicology·D E Feierman, A I Cederbaum
Oct 1, 1988·Journal of Occupational Medicine. : Official Publication of the Industrial Medical Association·J L ChenS Pell
Jul 1, 1988·Toxicology Letters·R TandonS P Srivastava
Jan 1, 1985·Critical Reviews in Toxicology·F P Guengerich, D C Liebler
Dec 1, 1983·Chemico-biological Interactions·M Y Farooqui, A E Ahmed
Jan 1, 1993·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·R A WardH J Gurland
Jan 1, 1993·Food Additives and Contaminants·H B ConacherJ J Ryan
Apr 1, 1993·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·A M SaillenfaitJ de Ceaurriz

❮ Previous
Next ❯

Citations

Aug 6, 1999·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·A M MostafaF M Hamada
Nov 20, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Hamdy A A AlyAshraf B Abdel-Naim

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Related Papers

Pharmacological Research : the Official Journal of the Italian Pharmacological Society
A M MostafaF M Hamada
Drug Metabolism and Disposition : the Biological Fate of Chemicals
M E Abreu, A E Ahmed
Research Communications in Chemical Pathology and Pharmacology
M Y FarooquiJ Piper
Journal of Biochemical Toxicology
M Y FarooquiR Cavazos
Drug Metabolism and Disposition : the Biological Fate of Chemicals
A E AhmedM W Anders
© 2021 Meta ULC. All rights reserved