In vitro testing of endothelial cell monolayers under dynamic conditions inside a beating ventricular prosthesis

ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs
V NikolaychikP I Lelkes

Abstract

Thromboembolic complications remain a major problem associated with the long-term clinical use of cardiac prostheses. A promising approach toward resolving this predicament is lining the blood contacting surfaces with a functional monolayer of endothelial cells (EC). In developing an endothelialized cardiac prosthesis, the authors in the past focused on establishing a confluent EC monolayer on the luminal surface of ventricular blood sacs. In this study, the authors concentrated on exposing the post confluent monolayers to the dynamic conditions inside a beating ventricle. The cells, derived from either bovine aortae or jugular veins, were grown to post confluence inside fully assembled ventricles on fibronectin or plasma cryoprecipitate coated, textured surfaces. After 11 days of culturing under static conditions, the endothelialized ventricles were connected to a mock loop that was run for 6 and 24 hr at 60 bpm and mean flow rate of 3.2 L/min. The status of the monolayer was evaluated by Alamar Blue assay before and after each run, and the extent of surface coverage was determined visually using bright field microscopic study after cell staining with KMnO4 and toluidine blue. In addition, morphometric information on cells/pol...Continue Reading

Citations

May 17, 2005·Biomaterials·Mengyan LiPeter I Lelkes
Dec 1, 1998·Endothelium : Journal of Endothelial Cell Research·K KandaP I Lelkes
Sep 2, 2006·Journal of Biomedical Materials Research. Part a·Mengyan LiPeter I Lelkes
Dec 25, 2008·Expert Review of Medical Devices·Dong-Choon SinXigeng Miao
Sep 10, 2020·Annals of Biomedical Engineering·Aldo FerrariEdoardo Mazza

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